Protein engineering design from directed evolution to de novo synthesis
With the development of enzyme engineering technology, green enzyme catalysis is widely
poised to replace traditional chemical catalysis enabling high enantioselectivity and yielding …
poised to replace traditional chemical catalysis enabling high enantioselectivity and yielding …
Building enzymes through design and evolution
EJ Hossack, FJ Hardy, AP Green - ACS Catalysis, 2023 - ACS Publications
Designing efficient enzymes is a formidable challenge at the forefront of modern
biocatalysis. Here, we review recent developments in the field and illustrate how the …
biocatalysis. Here, we review recent developments in the field and illustrate how the …
Current advances in design and engineering strategies of industrial enzymes
T Dinmukhamed, Z Huang, Y Liu, X Lv, J Li… - Systems Microbiology …, 2021 - Springer
Enzymes are considered as functional proteins, also known as biocatalysts, which are
required for normal cellular function. The commercial production of enzymes with distinct …
required for normal cellular function. The commercial production of enzymes with distinct …
Machine learning-assisted enzyme engineering
NE Siedhoff, U Schwaneberg, MD Davari - Methods in enzymology, 2020 - Elsevier
Directed evolution and rational design are powerful strategies in protein engineering to tailor
enzyme properties to meet the demands in academia and industry. Traditional approaches …
enzyme properties to meet the demands in academia and industry. Traditional approaches …
Tailoring multipurpose biocatalysts via protein engineering approaches: a review
The current industrial revolution signifies the high-value of protein engineering. The
development of multipurpose biocatalysts is significantly expanding as a result of increased …
development of multipurpose biocatalysts is significantly expanding as a result of increased …
[HTML][HTML] Rational design of enzyme activity and enantioselectivity
The strategy of rational design to engineer enzymes is to predict the potential mutants based
on the understanding of the relationships between protein structure and function, and …
on the understanding of the relationships between protein structure and function, and …
The state-of-the-art strategies of protein engineering for enzyme stabilization
Q Liu, G Xun, Y Feng - Biotechnology advances, 2019 - Elsevier
Enzymes generated by natural recruitment and protein engineering have greatly contribute
in various sets of applications. However, their insufficient stability is a bottleneck that limit the …
in various sets of applications. However, their insufficient stability is a bottleneck that limit the …
Artificial enzymes bringing together computational design and directed evolution
B de Pina Mariz, S Carvalho, IL Batalha… - Organic & Biomolecular …, 2021 - pubs.rsc.org
Enzymes are proteins that catalyse chemical reactions and, as such, have been widely used
to facilitate a variety of natural and industrial processes, dating back to ancient times. In fact …
to facilitate a variety of natural and industrial processes, dating back to ancient times. In fact …
Revolutionizing enzyme engineering through artificial intelligence and machine learning
The combinatorial space of an enzyme sequence has astronomical possibilities and
exploring it with contemporary experimental techniques is arduous and often ineffective …
exploring it with contemporary experimental techniques is arduous and often ineffective …
De novo design of biocatalysts
The challenging field of de novo enzyme design is beginning to produce exciting results.
The application of powerful computational methods to functional protein design has recently …
The application of powerful computational methods to functional protein design has recently …