Quantitative monitoring of autophagic degradation

A Kawai, S Takano, N Nakamura, S Ohkuma - Biochemical and biophysical …, 2006 - Elsevier
We developed a quantitative method for analyzing the induction of autophagy using a CHO-
K1 cell line stably expressing a green fluorescent protein (GFP) in mitochondrial matrix …

A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications

S Guo, Y Liang, SF Murphy, A Huang, H Shen… - Autophagy, 2015 - Taylor & Francis
The lack of a rapid and quantitative autophagy assay has substantially hindered the
development and implementation of autophagy-targeting therapies for a variety of human …

[PDF][PDF] Development of small fluorescent probes for the analysis of autophagy kinetics

HT Sakurai, H Iwashita, S Arakawa, A Yikelamu… - Iscience, 2023 - cell.com
Autophagy is a dynamic process that degrades subcellular constituents, and its activity is
measured by autophagic flux. The tandem proteins RFP-GFP-LC3 and GFP-LC3-RFP …

[HTML][HTML] Identifying specific receptors for cargo-mediated autophagy

M Goodall, A Thorburn - Cell research, 2014 - nature.com
Autophagy is a cell survival process whereby double-membrane structures,
autophagosomes, sequester bulk cytoplasm, damaged proteins, and organelles for delivery …

Methods for detecting autophagy and determining autophagy-induced cell death

Y Chen, MB Azad, SB Gibson - Canadian journal of physiology …, 2010 - cdnsciencepub.com
Autophagy is an intracellular lysosomal degradation process, which in the case of
macroautophagy, is characterized by the formation of double-membraned autophagosomes …

Human autophagy measurement: an underappreciated barrier to translation

TJ Sargeant, J Bensalem - Trends in Molecular Medicine, 2021 - cell.com
Preclinical research shows that autophagy is a modifiable process that holds promise for
preventing human age-related disease. However, this knowledge has not been clinically …

Quantitation of autophagy by luciferase release assay

R Ketteler, B Seed - Autophagy, 2008 - Taylor & Francis
Autophagy is a cellular process that has been defined and analyzed almost entirely by
qualitative measures. In no small part, this is attributable to the absence of robust …

[HTML][HTML] Assays to monitor autophagy progression in cell cultures

I Orhon, F Reggiori - Cells, 2017 - mdpi.com
The vast number of implications of autophagy in multiple areas of life sciences and medicine
has attracted the interest of numerous scientists that aim to unveil the role of this process in …

A novel quantitative flow cytometry-based assay for autophagy

KE Eng, MD Panas, GBK Hedestam, GM McInerney - Autophagy, 2010 - Taylor & Francis
Autophagy is a cellular degradation process with an increasingly recognised importance in
many biological pathways such as nutrient sensing, stress responses and development. We …

[PDF][PDF] Unraveling the role of autophagy in cancer

B Levine - Autophagy, 2006 - Taylor & Francis
Unraveling the Role of Autophagy in Cancer Page 1 ©2005 LANDES BIOSCIENCE. DO
NOT DISTRIBUTE. www.landesbioscience.com Autophagy 65 [Autophagy 2:2, 65-66, April/May/June …