The yeast S. cerevisiae serves as a model organism for many decades. Numerous
molecular tools have been developed throughout the years to engineer its genome …

Saccharomyces cerevisiae continues to serve as a powerful model system for both basic
biological research and industrial application. The development of genome-wide collections …

Saccharomyces cerevisiae has been for a long time a common model for fundamental
biological studies and a popular biotechnological engineering platform to produce …

One remarkable achievement in synthetic biology was the reconstruction of mycoplasma
genomes and their cloning in yeast where they can be modified using available genetic …

A variety of techniques for strain engineering in Saccharomyces cerevisiae have recently
been developed. However, especially when multiple genetic manipulations are required …

Yeasts represent a group of the microorganisms most frequently seen in biotechnology.
Recently, the class 2 type II CRISPR system (CRISPR/Cas9) has become the principal …

The genetic engineering of genome fragments larger than 100 kbp is challenging and
requires both specific methods and cloning hosts. The yeast Saccharomyces cerevisiae is …

CRISPR‐Cas9 has emerged as a powerful method for editing the genome in a wide variety
of species, since it can generate a specific DNA break when targeted by the Cas9‐bound …

The recent discovery and use of CRISPR/Cas9 gene editing technology has provided new
opportunities for scientific research in many fields of study including agriculture, genetic …

CRISPR-Cas9 technology is widely used for precise and specific editing of Saccharomyces
cerevisiae genome to obtain marker-free engineered hosts. Targeted double-strand breaks …