TadA orthologs enable both cytosine and adenine editing of base editors
S Zhang, B Yuan, J Cao, L Song, J Chen, J Qiu… - Nature …, 2023 - nature.com
Cytidine and adenosine deaminases are required for cytosine and adenine editing of base
editors respectively, and no single deaminase could enable concurrent and comparable …
editors respectively, and no single deaminase could enable concurrent and comparable …
Improved cytosine base editors generated from TadA variants
Cytosine base editors (CBEs) enable programmable genomic C· G-to-T· A transition
mutations and typically comprise a modified CRISPR–Cas enzyme, a naturally occurring …
mutations and typically comprise a modified CRISPR–Cas enzyme, a naturally occurring …
Re-engineering the adenine deaminase TadA-8e for efficient and specific CRISPR-based cytosine base editing
Cytosine base editors (CBEs) efficiently generate precise C· G-to-T· A base conversions, but
the activation-induced cytidine deaminase/apolipoprotein B mRNA-editing enzyme catalytic …
the activation-induced cytidine deaminase/apolipoprotein B mRNA-editing enzyme catalytic …
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity
Cytosine base editors (CBEs) are larger and can suffer from higher off-target activity or lower
on-target editing efficiency than current adenine base editors (ABEs). To develop a CBE that …
on-target editing efficiency than current adenine base editors (ABEs). To develop a CBE that …
Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Applications of adenine base editors (ABEs) have been constrained by the limited
compatibility of the deoxyadenosine deaminase component with Cas homologs other than …
compatibility of the deoxyadenosine deaminase component with Cas homologs other than …
An adenine base editor variant expands context compatibility
Adenine base editors (ABEs) are precise gene-editing agents that convert A: T pairs into G:
C through a deoxyinosine intermediate. Existing ABEs function most effectively when the …
C through a deoxyinosine intermediate. Existing ABEs function most effectively when the …
Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants
L Yang, X Zhang, L Wang, S Yin, B Zhu, L Xie… - Protein & …, 2018 - academic.oup.com
The clustered regularly interspaced short palindromic repeat (CRISPR) system has been
widely adapted to genome editing to either introduce or correct genetic mutations (Wang et …
widely adapted to genome editing to either introduce or correct genetic mutations (Wang et …
Docking sites inside Cas9 for adenine base editing diversification and RNA off-target elimination
S Li, B Yuan, J Cao, J Chen, J Chen, J Qiu… - Nature …, 2020 - nature.com
Base editing tools with diversified editing scopes and minimized RNA off-target activities are
required for broad applications. Nevertheless, current Streptococcus pyogenes Cas9 …
required for broad applications. Nevertheless, current Streptococcus pyogenes Cas9 …
A split and inducible adenine base editor for precise in vivo base editing
DNA base editors use deaminases fused to a programmable DNA-binding protein for
targeted nucleotide conversion. However, the most widely used TadA deaminases lack post …
targeted nucleotide conversion. However, the most widely used TadA deaminases lack post …
Phage-assisted evolution of highly active cytosine base editors with enhanced selectivity and minimal sequence context preference
E Zhang, ME Neugebauer, NA Krasnow… - Nature …, 2024 - nature.com
TadA-derived cytosine base editors (TadCBEs) enable programmable C• G-to-T• A editing
while retaining the small size, high on-target activity, and low off-target activity of TadA …
while retaining the small size, high on-target activity, and low off-target activity of TadA …