Highly efficient base editing in human tripronuclear zygotes
There are hundreds of disease-causing single-gene mutations, mainly caused by single-
nucleotide substitutions or point mutations rather than small insertions/deletions (indels) …
nucleotide substitutions or point mutations rather than small insertions/deletions (indels) …
BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity
Base editor (BE), containing a cytidine deaminase and catalytically defective Cas9, has
been widely used to perform base editing. However, the narrow editing window of BE limits …
been widely used to perform base editing. However, the narrow editing window of BE limits …
Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors
Cytosine base editors (CBEs) enable targeted C• G-to-T• A conversions in genomic DNA.
Recent studies report that BE3, the original CBE, induces a low frequency of genome-wide …
Recent studies report that BE3, the original CBE, induces a low frequency of genome-wide …
Computer simulations explain mutation-induced effects on the DNA editing by adenine base editors
Adenine base editors, which were developed by engineering a transfer RNA adenosine
deaminase enzyme (TadA) into a DNA editing enzyme (TadA*), enable precise modification …
deaminase enzyme (TadA) into a DNA editing enzyme (TadA*), enable precise modification …
Off-Targeting of Base Editors: BE3 but not ABE induces substantial off-target single nucleotide variants
H Xin, T Wan, Y Ping - Signal transduction and targeted therapy, 2019 - nature.com
Two recent articles 1, 2 published in Science separately reported, for the first time, that a
cytosine base editor but not an adenine base editor could induce considerable off-target …
cytosine base editor but not an adenine base editor could induce considerable off-target …
TadA reprogramming to generate potent miniature base editors with high precision
S Zhang, L Song, B Yuan, C Zhang, J Cao… - Nature …, 2023 - nature.com
Although miniature CRISPR-Cas12f systems were recently developed, the editing efficacy
and targeting range of derived miniature cytosine and adenine base editors (miniCBEs and …
and targeting range of derived miniature cytosine and adenine base editors (miniCBEs and …
Human cell based directed evolution of adenine base editors with improved efficiency
J Fu, Q Li, X Liu, T Tu, X Lv, X Yin, J Lv, Z Song… - Nature …, 2021 - nature.com
Adenine base editors (ABE) are genome-editing tools that have been harnessed to
introduce precise A• T to G• C conversion. However, the low activity of ABE at certain sites …
introduce precise A• T to G• C conversion. However, the low activity of ABE at certain sites …
CRISPR adenine and cytosine base editors with reduced RNA off-target activities
CRISPR-guided DNA base editors enable the efficient installation of targeted single-
nucleotide changes. Cytosine or adenine base editors (CBE s or ABE s), which are fusions …
nucleotide changes. Cytosine or adenine base editors (CBE s or ABE s), which are fusions …
Deep learning models incorporating endogenous factors beyond DNA sequences improve the prediction accuracy of base editing outcomes
T Yuan, L Wu, S Li, J Zheng, N Li, X Xiao, H Zhang… - Cell Discovery, 2024 - nature.com
Adenine base editors (ABEs) and cytosine base editors (CBEs) enable the single nucleotide
editing of targeted DNA sites avoiding generation of double strand breaks, however, the …
editing of targeted DNA sites avoiding generation of double strand breaks, however, the …
Cas12a base editors induce efficient and specific editing with low DNA damage response
X Wang, C Ding, W Yu, Y Wang, S He, B Yang… - Cell reports, 2020 - cell.com
The advent of base editors (BEs) holds great potential for correcting pathogenic-related
point mutations to treat relevant diseases. However, Cas9 nickase (nCas9)-derived BEs …
point mutations to treat relevant diseases. However, Cas9 nickase (nCas9)-derived BEs …