Cytosine base editing systems with minimized off-target effect and molecular size
A Li, H Mitsunobu, S Yoshioka, T Suzuki… - Nature …, 2022 - nature.com
Cytosine base editing enables the installation of specific point mutations without double-
strand breaks in DNA and is advantageous for various applications such as gene therapy …
strand breaks in DNA and is advantageous for various applications such as gene therapy …
Examination of the cell cycle dependence of cytosine and adenine base editors
CA Burnett, AT Wong, CA Vasquez… - Frontiers in Genome …, 2022 - frontiersin.org
Base editors (BEs) are genome editing agents that install point mutations with high efficiency
and specificity. Due to their reliance on uracil and inosine DNA damage intermediates …
and specificity. Due to their reliance on uracil and inosine DNA damage intermediates …
Eliminating base-editor-induced genome-wide and transcriptome-wide off-target mutations
L Wang, W Xue, H Zhang, R Gao, H Qiu, J Wei… - Nature Cell …, 2021 - nature.com
The fusion of CRISPR–Cas9 with cytidine deaminases leads to base editors (BEs) capable
of programmable C-to-T editing, which has potential in clinical applications but suffers from …
of programmable C-to-T editing, which has potential in clinical applications but suffers from …
A cytosine base editor toolkit with varying activity windows and target scopes for versatile gene manipulation in plants
X Xiong, Z Li, J Liang, K Liu, C Li… - Nucleic Acids Research, 2022 - academic.oup.com
CRISPR/Cas-derived base editing tools empower efficient alteration of genomic cytosines or
adenines associated with essential genetic traits in plants and animals. Diversified target …
adenines associated with essential genetic traits in plants and animals. Diversified target …
ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems
J Xie, X Huang, X Wang, S Gou, Y Liang, F Chen, N Li… - BMC biology, 2020 - Springer
Background Many favorable traits of crops and livestock and human genetic diseases arise
from multiple single nucleotide polymorphisms or multiple point mutations with …
from multiple single nucleotide polymorphisms or multiple point mutations with …
Base editors: development and applications in biomedicine
Y Liang, F Chen, K Wang, L Lai - Frontiers of Medicine, 2023 - Springer
Base editor (BE) is a gene-editing tool developed by combining the CRISPR/Cas system
with an individual deaminase, enabling precise single-base substitution in DNA or RNA …
with an individual deaminase, enabling precise single-base substitution in DNA or RNA …
Genome-wide profiling of adenine base editor specificity by EndoV-seq
P Liang, X Xie, S Zhi, H Sun, X Zhang, Y Chen… - Nature …, 2019 - nature.com
The adenine base editor (ABE), capable of catalyzing A• T to G• C conversions, is an
important gene editing toolbox. Here, we systematically evaluate genome-wide off-target …
important gene editing toolbox. Here, we systematically evaluate genome-wide off-target …
Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor
L Wang, W Xue, L Yan, X Li, J Wei, M Chen, J Wu… - Cell Research, 2017 - nature.com
Base editors (BEs) have been recently developed by combining the APOBEC
(apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like)/AID (activation-induced …
(apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like)/AID (activation-induced …
PhieCBEs: plant high-efficiency cytidine base editors with expanded target range
D Zeng, T Liu, J Tan, Y Zhang, Z Zheng, B Wang… - Molecular Plant, 2020 - cell.com
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
protein 9 (Cas9)-mediated genome editing can efficiently produce gene-knockout mutants …
protein 9 (Cas9)-mediated genome editing can efficiently produce gene-knockout mutants …
Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors
Base editing requires that the target sequence satisfy the protospacer adjacent motif
requirement of the Cas9 domain and that the target nucleotide be located within the editing …
requirement of the Cas9 domain and that the target nucleotide be located within the editing …