[HTML][HTML] Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors
F Chen, M Lian, B Ma, S Gou, X Luo, K Yang… - Communications …, 2022 - nature.com
Cas12a can process multiple sgRNAs from a single transcript of CRISPR array, conferring
advantages in multiplexed base editing when incorporated into base editor systems, which …
advantages in multiplexed base editing when incorporated into base editor systems, which …
APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels
AE Rieffer, Y Chen, DJ Salamango, SN Moraes… - The CRISPR …, 2023 - liebertpub.com
Precision genome editing has become a reality with the discovery of base editors. Cytosine
base editor (CBE) technologies are improving rapidly but are mostly optimized for TC …
base editor (CBE) technologies are improving rapidly but are mostly optimized for TC …
[HTML][HTML] Base editors: expanding the types of DNA damage products harnessed for genome editing
Base editors are an innovative addition to the genome editing toolbox that introduced a new
genome editing strategy to the field. Instead of using double-stranded DNA breaks, base …
genome editing strategy to the field. Instead of using double-stranded DNA breaks, base …
Design and application of the transformer base editor in mammalian cells and mice
Fusing apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like cytidine
deaminase with catalytically impaired Cas proteins (eg, nCas9 or dCas9) provides a novel …
deaminase with catalytically impaired Cas proteins (eg, nCas9 or dCas9) provides a novel …
[HTML][HTML] Rationally designed APOBEC3B cytosine base editors with improved specificity
Cytosine base editors (CBEs) generate C-to-T nucleotide substitutions in genomic target
sites without inducing double-strand breaks. However, CBEs such as BE3 can cause …
sites without inducing double-strand breaks. However, CBEs such as BE3 can cause …
[HTML][HTML] A precise and efficient adenine base editor
T Tu, Z Song, X Liu, S Wang, X He, H Xi, J Wang… - Molecular Therapy, 2022 - cell.com
Adenine base editors (ABEs) are novel genome-editing tools, and their activity has been
greatly enhanced by eight additional mutations, thus named ABE8e. However, elevated …
greatly enhanced by eight additional mutations, thus named ABE8e. However, elevated …
[HTML][HTML] Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing
J Fan, Y Ding, C Ren, Z Song, J Yuan, Q Chen… - Communications …, 2021 - nature.com
Cytosine or adenine base editors (CBEs or ABEs) hold great promise in therapeutic
applications because they enable the precise conversion of targeted base changes without …
applications because they enable the precise conversion of targeted base changes without …
Efficient and high-fidelity base editor with expanded PAM compatibility for cytidine dinucleotide
Cytidine base editor (CBE), which is composed of a cytidine deaminase fused to Cas9
nickase, has been widely used to induce C-to-T conversions in a wide range of organisms …
nickase, has been widely used to induce C-to-T conversions in a wide range of organisms …
CRISPR DNA base editors with reduced RNA off-target and self-editing activities
Cytosine or adenine base editors (CBEs or ABEs) can introduce specific DNA C-to-T or A-to-
G alterations,,–. However, we recently demonstrated that they can also induce transcriptome …
G alterations,,–. However, we recently demonstrated that they can also induce transcriptome …
[HTML][HTML] Compact zinc finger architecture utilizing toxin-derived cytidine deaminases for highly efficient base editing in human cells
Nucleobase editors represent an emerging technology that enables precise single-base
edits to the genomes of eukaryotic cells. Most nucleobase editors use deaminase domains …
edits to the genomes of eukaryotic cells. Most nucleobase editors use deaminase domains …