The low efficiency in obtaining piglets after production of cloned embryos was challenged in
two steps—first by performing in vitro culture for 5–6 days after cloning to obtain later-stage …

The efficiency of porcine somatic nuclear transfer (born piglets/transferred embryos) is low.
Here, we report a highly efficient protocol using peripubertal gilts as recipients synchronized …

Nuclear transfer (NT) is a procedure by which genetically identical individuals can be
created. The applications of these NT techniques will be in agriculture, biomedicine, and …

Currently, cloning efficiency in pigs is very low. Donor cell type and number of cloned
embryos transferred to an individual surrogate are two major factors that affect the …

The birth of the first nuclear transfer-derived cloned piglet resulted from the transfer of a
nucleus from a 4-cell stage embryo to an enucleated oocyte. Although biotechnological …

NUCLEAR TRANSFER is a procedure by which genetically identical individuals can be
created. The applications of these techniques for nuclear transfer will be in agricultural …

Cloning of Pigs from Somatic Cells and Its Prospects Page 1 CLONING AND STEM CELLS
Volume 4, Number 3, 2002 © Mary Ann Liebert, Inc. Cloning of Pigs from Somatic Cells and Its …

Here we describe a procedure for cloning pigs by the use of in vitro culture systems. Four
healthy male piglets from two litters were born following nuclear transfer of cultured somatic …

To date, the efficiency of pig cloning by nuclear transfer of somatic cell nuclei has been
extremely low, with less than 1% of transferred embryos surviving to term. Even the …

Although mouse embryonic stem cells have been used widely for over a decade as an
important tool for introducing precise genetic modification into the genome, demonstrating …