Pig transgenesis by piggyBac transposition in combination with somatic cell nuclear transfer

Z Wu, Z Xu, X Zou, F Zeng, J Shi, D Liu, J Urschitz… - Transgenic …, 2013 - Springer
The production of animals by somatic cell nuclear transfer (SCNT) is inefficient, with
approximately 2% of micromanipulated oocytes going to term and resulting in live births …

Production of cloned pigs by using somatic cells as donors

L Lai, RS Prather - Cloning & Stem Cells, 2003 - liebertpub.com
NUCLEAR TRANSFER is a procedure by which genetically identical individuals can be
created. The applications of these techniques for nuclear transfer will be in agricultural …

Nuclear transfer and transgenesis in the pig

M Kurome, B Kessler, A Wuensch… - … : Methods and Protocols, 2015 - Springer
Somatic cell nuclear transfer (SCNT) using genetically modified donor cells facilitates the
generation of tailored pig models for biomedical research and for xenotransplantation. Up to …

[HTML][HTML] The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs

M Sato, K Maeda, M Koriyama, E Inada… - International Journal of …, 2016 - mdpi.com
The introduction of multigene constructs into single cells is important for improving the
performance of domestic animals, as well as understanding basic biological processes. In …

[HTML][HTML] Generation of bovine transgenics using somatic cell nuclear transfer

CA Hodges, SL Stice - Reproductive Biology and Endocrinology, 2003 - Springer
The ability to produce transgenic animals through the introduction of exogenous DNA has
existed for many years. However, past methods available to generate transgenic animals …

A Highly Efficient Method for Porcine Cloning by Nuclear Transfer Using In Vitro-Matured Oocytes

SC Walker, T Shin, GM Zaunbrecher… - Cloning & Stem …, 2002 - liebertpub.com
To date, the efficiency of pig cloning by nuclear transfer of somatic cell nuclei has been
extremely low, with less than 1% of transferred embryos surviving to term. Even the …

Pig cloning using somatic cell nuclear transfer

H Ouyang, J Han, Y Huang - Nuclear Reprogramming: Methods and …, 2021 - Springer
Porcine cloning technology can be used to produce progenies genetically identical to the
donor cells from high-quality breeding pigs. In addition, genetically modified pigs have been …

A method for producing cloned pigs by using somatic cells as donors

L Lai, RS Prather - Germ Cell Protocols: Volume 2: Molecular Embryo …, 2004 - Springer
Nuclear transfer (NT) is a procedure by which genetically identical individuals can be
created. The applications of these NT techniques will be in agriculture, biomedicine, and …

Cloning pigs: advances and applications.

IA Polejaeva - Reproduction (Cambridge, England). Supplement, 2001 - europepmc.org
Although mouse embryonic stem cells have been used widely for over a decade as an
important tool for introducing precise genetic modification into the genome, demonstrating …

[PDF][PDF] Generation of transgenic livestock by somatic cell nuclear transfer

P Bosch, CA Hodges, SL Stice - Biotecnología Aplicada, 2004 - biblat.unam.mx
The ability to produce transgenic animals through the introduction of exogenous DNA has
existed for many years. However, past methods available to generate transgenic animals …