The enzyme hydrogenase, from the photosyn-thetic bacterium Chromatium, was purified to
homogeneity after solubilization of the particulate enzyme with deoxy-cholate. The …

The kinetics of paraH2-orthoH2 conversion and the HD exchange reactions catalyzed by
highly purified desulfovibrio hydrogenase [H2: ferricytochrome c 3 oxidoreductase] were …

Previous reports of a relatively air-stable soluble hydrogenase from the photosynthetic
anaerobe, Chromatium vinosum, have been substantiated. The properties of this enzyme, as …

The reaction takes place at a specialized metal center that dramatically increases the acidity
of H2 and leads to a heterolytic splitting of the molecule which is strongly accelerated by the …

The specific activity and the EPR spectra of purified hydrogenase from Chromatium vinosum
vary from preparation to preparation. It has been found that both properties are dependent …

The kinetics of hydrogenase from Clostridium pasteurianum and from Proteus vulgaris were
studied by measuring the HD and D/sub 2/produced when the enzyme catalyzes the …

A routine procedure for the growth and harvesting of large (600 1) batches of Chromatium
vinosum and the isolation of hydrogenase (hydrogen:(acceptor) oxidoreductase, EC …

Hydrogenase from the marine green alga, Chlorococcum littorale, was purified 1485‐fold,
resulting in a specific activity for hydrogen evolution of 75.7 μmol/min/mg of protein at 25° C …

The oxidized form of hydrogen dehydrogenase of Hydrogenomonas ruhlandii was inhibited
by BAL, cyanide, dithionite, sulfide, sulfite and thioglycolate. Reduction of the …

The membrane-bound hydrogenase of the photosynthetic bacterium Rhodospirillum rubrum
has been purified 490-fold with a yield of 5.8%. The enzyme was homogeneous by disc gel …