This report describes the design, construction, and expression of a bivalent bispecific single-
chain antibody (SCA) protein in Escherichia coli. The bispecificity of the bivalent protein was …

The specificity of the interaction between the combining site (paratope) of an antibody
molecule and an antigenic determinant (epitope) is a concept originated from the first …

Natural polyreactive autoantibodies (NAA) are an important component of the normal B cell
repertoire. One intriguing characteristic of these Abs is their binding to various dissimilar …

The three-dimensional structure of several Fab fragments from human immunoglobulins and
murine monoclonal antibodies (MAbs) have been determined by X-ray crystallography (for …

Background: Diabodies are dimeric antibody fragments. In each polypeptide, a heavy-chain
variable domain (VH) is linked to a light-chain variable domain (VL) but unlike single-chain …

We have determined the three-dimensional structures of the antigen-binding fragment of the
anti-digoxin monoclonal antibody 26-10 in the uncomplexed state at 2.7 A resolution and as …

The ability of immunoglobulins to recognize and adapt to antigenic determinants provides a
flexible and refined system for ensuring the specificity of the immune response. In recent …

A gene encoding mouse-human chimeric secreted IgD was constructed using the
rearranged murine variable region specific for the hapten dansyl and the genomic gene …

The Role of the Inter-heavy Chain Disulfide Bond in Modulating the Flexibility of lmmunoglobulin
G Antibody Page 1 J. Mol. Biol. (1977) 112, 653-656 The Role of the Inter-heavy Chain …

Changes in the quantity and quality of antibodies occur in the course of an immune
response. This review describes the physicochemical and biological properties of …