The development of next generation sequencing (NGS) techniques has enabled
researchers to study and understand the world of microorganisms from broader and deeper …

Background The new field of metagenomics studies microorganism communities by culture-
independent sequencing. With the advances in next-generation sequencing techniques …

The amount of host DNA poses a major challenge to metagenome analysis. However, there
is no guidance on the levels of host DNA, nor on the depth of sequencing needed to acquire …

Characterization of the bacterial composition and functional repertoires of microbiome
samples is the most common application of metagenomics. Although deep whole …

The complexity of microbial communities, combined with technical biases in next-generation
sequencing, pose a challenge to metagenomic analysis. Here, we develop a set of internal …

The study of microorganisms that pervade each and every part of this planet has
encountered many challenges through time such as the discovery of unknown organisms …

New sequencing methods generate data that can allow the assembly of microbial genome
sequences in days. With such revolutionary advances in technology come new challenges …

The improvement of sequencing technologies has facilitated generalization of metagenomic
sequencing, which has become a standard procedure for analyzing the structure and …

The application of shotgun sequencing to environmental samples has revealed a new
universe of microbial community genomes (metagenomes) involving previously uncultured …

The development and continuous improvement of high-throughput sequencing platforms
have stimulated interest in the study of complex microbial communities. Currently, the most …