Investigation of the pKa of the Nucleophilic O2′ of the Hairpin Ribozyme
AJ Veenis, P Li, AV Soudackov… - The Journal of …, 2021 - ACS Publications
Small ribozymes cleave their RNA phosphodiester backbone by catalyzing a
transphosphorylation reaction wherein a specific O2′ functions as the nucleophile. While …
transphosphorylation reaction wherein a specific O2′ functions as the nucleophile. While …
Metal ions in the structure and function of RNA
A Pyle - JBIC Journal of Biological Inorganic Chemistry, 2002 - Springer
It is becoming increasingly clear that RNA is more than a passive carrier of genetic
information. Folded RNA molecules play key roles in almost every aspect of cellular …
information. Folded RNA molecules play key roles in almost every aspect of cellular …
Evidence for a catalytic strategy to promote nucleophile activation in metal-dependent RNA-cleaving ribozymes and 8-17 DNAzyme
An unique catalytic strategy was recently reported for the glm S ribozyme [Bingaman et al.,
Nat. Chem. Biol. 2017, 13, 439− 445] that involves promotion of productive hydrogen …
Nat. Chem. Biol. 2017, 13, 439− 445] that involves promotion of productive hydrogen …
Multiple roles of metal ions in large ribozymes
D Donghi, J Schnabl - 2011 - books.rsc.org
Since the discovery of catalytic RNA molecules (ribozymes), intense research has been
devoted to understand their structure and activity. Among RNA molecules, the large …
devoted to understand their structure and activity. Among RNA molecules, the large …
The catalytic diversity of RNAs
MJ Fedor, JR Williamson - Nature reviews Molecular cell biology, 2005 - nature.com
The natural RNA enzymes catalyse phosphate-group transfer and peptide-bond formation.
Initially, metal ions were proposed to supply the chemical versatility that nucleotides lack. In …
Initially, metal ions were proposed to supply the chemical versatility that nucleotides lack. In …
Metal-ion rescue revisited: biochemical detection of site-bound metal ions important for RNA folding
Within the three-dimensional architectures of RNA molecules, divalent metal ions populate
specific locations, shedding their water molecules to form chelates. These interactions help …
specific locations, shedding their water molecules to form chelates. These interactions help …
NMR Localization of Divalent Cations at the Active Site of the Neurospora VS Ribozyme Provides Insights into RNA–Metal-Ion Interactions
E Bonneau, P Legault - Biochemistry, 2014 - ACS Publications
Metal cations represent key elements of RNA structure and function. In the Neurospora VS
ribozyme, metal cations play diverse roles; they are important for substrate recognition …
ribozyme, metal cations play diverse roles; they are important for substrate recognition …
The hammerhead, hairpin and VS ribozymes are catalytically proficient in monovalent cations alone
Background: The catalytic activity of RNA enzymes is thought to require divalent metal ions,
which are believed to facilitate RNA folding and to play a direct chemical role in the reaction …
which are believed to facilitate RNA folding and to play a direct chemical role in the reaction …
Establishing the catalytic and regulatory mechanism of RNA‐based machineries
Ribonucleoprotein (RNP)‐machineries are comprised of intricate networks of long
noncoding RNAs and proteins that allow them to actively participate in transcription, RNA …
noncoding RNAs and proteins that allow them to actively participate in transcription, RNA …
Structure determination of catalytic RNAs and investigations of their metal ion-binding properties
MC Erat, RKO Sigel - Chimia, 2005 - chimia.ch
Naturally occurring RNA molecules exhibit many unexpected and fascinating properties in
living cells such as protein synthesis and transport, regulation of metabolic functions, and …
living cells such as protein synthesis and transport, regulation of metabolic functions, and …