The hammerhead ribozyme is generally accepted as a well characterized metalloenzyme.
However, the precise nature of the interactions of the RNA with metal ions remains to be fully …

Electrostatic forces, acting between helices and modulated by the presence of the ion
atmosphere, are key determinants in the energetic balance that governs RNA folding …

Catalysis in RNA is intimately connected to the folding. The small nucleolytic ribozymes
function by a nucleophilic attack of the 2′-oxygen on the 3′-phosphate, in an SN 2 …

In pre-steady-state, fast-quench kinetic analysis, the tertiary-stabilized hammerhead
ribozyme “RzB” cleaves its substrate RNA with maximal measured k obs values of∼ 3000 …

Metal ions are required by all organisms in order to execute an array of essential molecular
functions. They play a critical role in many catalytic mechanisms and structural properties …

The folding and catalytic function of RNA molecules depend on their interactions with
divalent metal ions, such as magnesium. As with every molecular process, the most basic …

Nucleic acid enzymes require specific metal ions to be catalytically active. The functions of
the metal ions having structural and catalytic roles are affected by competing cations. Large …

To explore how an early, RNA-based life form could have functioned, in vitro selection
experiments have been used to develop catalytic RNAs (ribozymes) with relevant functions …

Translation–protein synthesis at the ribonucleic acid (RNA) based molecular machine, the
ribosome,–proceeds in a similar manner in all life forms. However, despite several decades …

The diverse functions of RNA provide numerous opportunities for programming biological
circuits. We describe a new strategy that uses ribozyme K28min to covalently tag a specific …