Efficient genome editing in wheat using Cas9 and Cpf1 (AsCpf1 and LbCpf1) nucleases

D Kim, M Hager, E Brant, H Budak - Functional & Integrative Genomics, 2021 - Springer
Genome editing can be used to create new wheat varieties with enhanced performance.
Clustered regularly interspaced short palindromic repeat (CRISPR) is a powerful tool for …

CRISPR‐Cas9 based genome editing in wheat

MA Smedley, S Hayta, M Clarke… - Current Protocols, 2021 - Wiley Online Library
The development and application of high precision genome editing tools such as
programmable nucleases are set to revolutionize crop breeding and are already having a …

Optimizing multiplex CRISPR/Cas9-based genome editing for wheat

W Wang, A Akhunova, S Chao, E Akhunov - BioRxiv, 2016 - biorxiv.org
Background CRISPR/Cas9-based genome editing holds great promise to accelerate the
development of improved crop varieties by providing a powerful tool to modify the genomic …

Development of an Agrobacterium‐delivered CRISPR/Cas9 system for wheat genome editing

Z Zhang, L Hua, A Gupta, D Tricoli… - Plant biotechnology …, 2019 - Wiley Online Library
CRISPR/Cas9 has been widely used for genome editing in many organisms, including
important crops like wheat. Despite the tractability in designing CRISPR/Cas9, efficacy in the …

CRISPR-Cas12a (Cpf1): a versatile tool in the plant genome editing tool box for agricultural advancement

A Bandyopadhyay, N Kancharla, VS Javalkote… - Frontiers in plant …, 2020 - frontiersin.org
Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion
from today. To meet the demands of growing, geographically and socio-economically …

[PDF][PDF] CRISPR/CasΦ2‐mediated gene editing in wheat and rye

S Zhao, X Han, Y Zhu, Y Han, H Liu… - Journal of Integrative …, 2024 - researchgate.net
The recent advancements in developing the CRISPR/Cas9 system and various derivative
tools (eg, base editors) have accelerated basic plant science research and crop …

[HTML][HTML] CRISPR-mediated acceleration of wheat improvement: advances and perspectives

X Zhou, Y Zhao, P Ni, Z Ni, Q Sun, Y Zong - Journal of genetics and …, 2023 - Elsevier
Common wheat (Triticum aestivum) is one of the most widely cultivated and consumed crops
globally. In the face of limited arable land and climate changes, it is a great challenge to …

Expanding the range of editable targets in the wheat genome using the variants of the Cas12a and Cas9 nucleases

W Wang, B Tian, Q Pan, Y Chen, F He… - Plant biotechnology …, 2021 - Wiley Online Library
The development of CRISPR‐based editors recognizing distinct protospacer‐adjacent
motifs (PAMs), or having different spacer length/structure requirements broadens the range …

Progresses of CRISPR/Cas9 genome editing in forage crops

SIU Haq, D Zheng, N Feng, X Jiang, F Qiao… - Journal of Plant …, 2022 - Elsevier
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-
associated protein 9 (Cas9) mediated-genome editing has evolved into a powerful tool that …

Updates on gene editing and its applications

H Puchta, J Jiang, K Wang, Y Zhao - Plant Physiology, 2022 - academic.oup.com
Recent advancements in gene editing provide unprecedented tools for plant biology
research and offer unlimited potential for improving existing crops and de novo …