MaxQuant for in-depth analysis of large SILAC datasets
Proteomics experiments can generate very large volumes of data, in particular in situations
where within one experimental design many samples are compared to each other, possibly …
where within one experimental design many samples are compared to each other, possibly …
NeuCode labels for relative protein quantification
We describe a synthesis strategy for the preparation of lysine isotopologues that differ in
mass by as little as 6 mDa. We demonstrate that incorporation of these molecules into the …
mass by as little as 6 mDa. We demonstrate that incorporation of these molecules into the …
Stable isotope-coded proteomic mass spectrometry
MB Goshe, RD Smith - Current opinion in biotechnology, 2003 - Elsevier
Developing the ability to quantify changes in protein abundance between cells subjected to
a variety of physiological and environmental conditions is an extremely active area of …
a variety of physiological and environmental conditions is an extremely active area of …
Quantitative proteome analysis by solid-phase isotope tagging and mass spectrometry
The adaptation of sequences of chemical reactions to a solid-phase format has been
essential to the automation, reproducibility, and efficiency of a number of biotechnological …
essential to the automation, reproducibility, and efficiency of a number of biotechnological …
Stable isotope labeling by amino acids in cell culture (SILAC) and proteome quantitation of mouse embryonic stem cells to a depth of 5,111 proteins
J Graumann, NC Hubner, JB Kim, K Ko, M Moser… - Molecular & Cellular …, 2008 - ASBMB
Embryonic stem (ES) cells are pluripotent cells isolated from mammalian preimplantation
embryos. They are capable of differentiating into all cell types and therefore hold great …
embryos. They are capable of differentiating into all cell types and therefore hold great …
A practical guide to the MaxQuant computational platform for SILAC-based quantitative proteomics
MaxQuant is a quantitative proteomics software package designed for analyzing large mass
spectrometric data sets. It is specifically aimed at high-resolution mass spectrometry (MS) …
spectrometric data sets. It is specifically aimed at high-resolution mass spectrometry (MS) …
Quantitative proteomics using SILAC: Principles, applications, and developments
X Chen, S Wei, Y Ji, X Guo, F Yang - Proteomics, 2015 - Wiley Online Library
SILAC is based on direct addition of selected stable isotope amino acids into the cell culture
medium, allowing superior quantitative analysis of the cellular proteome compared to other …
medium, allowing superior quantitative analysis of the cellular proteome compared to other …
Protein-based stable isotope probing
We describe a stable isotope probing (SIP) technique that was developed to link microbe-
specific metabolic function to phylogenetic information. Carbon (13C)-or nitrogen (15N) …
specific metabolic function to phylogenetic information. Carbon (13C)-or nitrogen (15N) …
Quantitative, time-resolved proteomic analysis by combining bioorthogonal noncanonical amino acid tagging and pulsed stable isotope labeling by amino acids in cell …
An approach to proteomic analysis that combines bioorthogonal noncanonical amino acid
tagging (BONCAT) and pulsed stable isotope labeling with amino acids in cell culture …
tagging (BONCAT) and pulsed stable isotope labeling with amino acids in cell culture …
Quantitative cancer proteomics: stable isotope labeling with amino acids in cell culture (SILAC) as a tool for prostate cancer research
PA Everley, J Krijgsveld, BR Zetter, SP Gygi - Molecular & Cellular …, 2004 - ASBMB
Microarrays have been the primary means for large-scale analyses of genes implicated in
cancer progression. However, more recently a need has been recognized for investigating …
cancer progression. However, more recently a need has been recognized for investigating …