Due to the complex nature of the proteome, instrumentation and methods development for
sample cleanup, fractionation, preconcentration, chromatographic separation and detection …

During the last ten to fifteen years a huge development in the field of analytical techniques
used for deciphering the complex nature of the proteome took place. Instrumentation and …

Proteomics, the characterization of the proteome, is conceptually simple but technically
challenging. Development of such technologies as mass spectrometry, multidimensional …

Multidimensional liquid-based separation techniques are described for maximizing the
resolution of the enormous number of peptides generated upon tryptic digestion of …

High-performance liquid chromatography–electrospray ionization tandem mass
spectrometry (HPLC–ESI-MS–MS) is regarded as one of the most powerful techniques for …

The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low
back pressure, easy preparation process and miniaturisation, and the availability of different …

This review provides an introduction for the nonspecialist to proteomics and in particular the
major approaches available for global protein identification and quantification. Proteomics …

Proteomics studies rely on high-resolving separation techniques whereby the biological
complexity can be unraveled. Both gel-based and liquid-phase separation principles are …

Proteomics is defined as the analysis of part or all of the protein components of a complex
biological system (a cell, organ or tissue) at a given moment. Due to the huge number of …

This review covers applications of separation and characterization of peptides and proteins
by liquid chromatography (LC), mass spectrometry (MS), Fourier transform infrared (FT-IR) …