High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate

BD Freibaum, J Messing, P Yang, HJ Kim… - Journal of Cell …, 2021 - rupress.org
Liquid–liquid phase separation (LLPS) is a mechanism of intracellular organization that
underlies the assembly of a variety of RNP granules. Fundamental biophysical principles …

[HTML][HTML] Competing protein-RNA interaction networks control multiphase intracellular organization

DW Sanders, N Kedersha, DSW Lee, AR Strom… - Cell, 2020 - cell.com
Liquid-liquid phase separation (LLPS) mediates formation of membraneless condensates
such as those associated with RNA processing, but the rules that dictate their assembly …

RNA droplets

K Rhine, V Vidaurre, S Myong - Annual review of biophysics, 2020 - annualreviews.org
Liquid–liquid phase separation is emerging as the universal mechanism by which
membraneless cellular granules form. Despite many previous studies on condensation of …

[HTML][HTML] Formation and maturation of phase-separated liquid droplets by RNA-binding proteins

Y Lin, DSW Protter, MK Rosen, R Parker - Molecular cell, 2015 - cell.com
Eukaryotic cells possess numerous dynamic membrane-less organelles, RNP granules,
enriched in RNA and RNA-binding proteins containing disordered regions. We demonstrate …

[HTML][HTML] RNA is a critical element for the sizing and the composition of phase-separated RNA–protein condensates

M Garcia-Jove Navarro, S Kashida, R Chouaib… - Nature …, 2019 - nature.com
Liquid–liquid phase separation is thought to be a key organizing principle in eukaryotic cells
to generate highly concentrated dynamic assemblies, such as the RNP granules. Numerous …

The liquid nucleome–phase transitions in the nucleus at a glance

AR Strom, CP Brangwynne - Journal of cell science, 2019 - journals.biologists.com
Cells organize membrane-less internal compartments through a process called liquid–liquid
phase separation (LLPS) to create chemically distinct compartments, referred to as …

Multiple modes of protein–protein interactions promote RNP granule assembly

T Mittag, R Parker - Journal of molecular biology, 2018 - Elsevier
Eukaryotic cells are known to contain a wide variety of RNA–protein assemblies, collectively
referred to as RNP granules. RNP granules form from a combination of RNA–RNA, protein …

[HTML][HTML] Coexisting liquid phases underlie nucleolar subcompartments

M Feric, N Vaidya, TS Harmon, DM Mitrea, L Zhu… - Cell, 2016 - cell.com
The nucleolus and other ribonucleoprotein (RNP) bodies are membrane-less organelles
that appear to assemble through phase separation of their molecular components. However …

[HTML][HTML] Who's in and who's out—compositional control of biomolecular condensates

JA Ditlev, LB Case, MK Rosen - Journal of molecular biology, 2018 - Elsevier
Biomolecular condensates are two-and three-dimensional compartments in eukaryotic cells
that concentrate specific collections of molecules without an encapsulating membrane …

[HTML][HTML] G3BP1 is a tunable switch that triggers phase separation to assemble stress granules

P Yang, C Mathieu, RM Kolaitis, P Zhang, J Messing… - Cell, 2020 - cell.com
The mechanisms underlying ribonucleoprotein (RNP) granule assembly, including the basis
for establishing and maintaining RNP granules with distinct composition, are unknown. One …