High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate
BD Freibaum, J Messing, P Yang, HJ Kim… - Journal of Cell …, 2021 - rupress.org
Liquid–liquid phase separation (LLPS) is a mechanism of intracellular organization that
underlies the assembly of a variety of RNP granules. Fundamental biophysical principles …
underlies the assembly of a variety of RNP granules. Fundamental biophysical principles …
[HTML][HTML] Competing protein-RNA interaction networks control multiphase intracellular organization
Liquid-liquid phase separation (LLPS) mediates formation of membraneless condensates
such as those associated with RNA processing, but the rules that dictate their assembly …
such as those associated with RNA processing, but the rules that dictate their assembly …
[HTML][HTML] Formation and maturation of phase-separated liquid droplets by RNA-binding proteins
Eukaryotic cells possess numerous dynamic membrane-less organelles, RNP granules,
enriched in RNA and RNA-binding proteins containing disordered regions. We demonstrate …
enriched in RNA and RNA-binding proteins containing disordered regions. We demonstrate …
[HTML][HTML] RNA is a critical element for the sizing and the composition of phase-separated RNA–protein condensates
M Garcia-Jove Navarro, S Kashida, R Chouaib… - Nature …, 2019 - nature.com
Liquid–liquid phase separation is thought to be a key organizing principle in eukaryotic cells
to generate highly concentrated dynamic assemblies, such as the RNP granules. Numerous …
to generate highly concentrated dynamic assemblies, such as the RNP granules. Numerous …
The liquid nucleome–phase transitions in the nucleus at a glance
AR Strom, CP Brangwynne - Journal of cell science, 2019 - journals.biologists.com
Cells organize membrane-less internal compartments through a process called liquid–liquid
phase separation (LLPS) to create chemically distinct compartments, referred to as …
phase separation (LLPS) to create chemically distinct compartments, referred to as …
Multiple modes of protein–protein interactions promote RNP granule assembly
Eukaryotic cells are known to contain a wide variety of RNA–protein assemblies, collectively
referred to as RNP granules. RNP granules form from a combination of RNA–RNA, protein …
referred to as RNP granules. RNP granules form from a combination of RNA–RNA, protein …
[HTML][HTML] Coexisting liquid phases underlie nucleolar subcompartments
The nucleolus and other ribonucleoprotein (RNP) bodies are membrane-less organelles
that appear to assemble through phase separation of their molecular components. However …
that appear to assemble through phase separation of their molecular components. However …
[HTML][HTML] Who's in and who's out—compositional control of biomolecular condensates
Biomolecular condensates are two-and three-dimensional compartments in eukaryotic cells
that concentrate specific collections of molecules without an encapsulating membrane …
that concentrate specific collections of molecules without an encapsulating membrane …
[HTML][HTML] G3BP1 is a tunable switch that triggers phase separation to assemble stress granules
The mechanisms underlying ribonucleoprotein (RNP) granule assembly, including the basis
for establishing and maintaining RNP granules with distinct composition, are unknown. One …
for establishing and maintaining RNP granules with distinct composition, are unknown. One …