Whole genome sequencing reveals rare off‐target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR/Cas9‐edited cotton plants

J Li, H Manghwar, L Sun, P Wang… - Plant biotechnology …, 2019 - Wiley Online Library
The CRISPR/Cas9 system has been extensively applied for crop improvement. However,
our understanding of Cas9 specificity is very limited in Cas9‐edited plants. To identify on …

[HTML][HTML] CRISPR for crop improvement: an update review

D Jaganathan, K Ramasamy, G Sellamuthu… - Frontiers in plant …, 2018 - frontiersin.org
The availability of genome sequences for several crops and advances in genome editing
approaches has opened up possibilities to breed for almost any given desirable trait …

[HTML][HTML] CRISPR/Cas9: a powerful tool for crop genome editing

G Song, M Jia, K Chen, X Kong, B Khattak, C Xie, A Li… - The crop journal, 2016 - Elsevier
The CRISPR/Cas9 technology is evolved from a type II bacterial immune system and
represents a new generation of targeted genome editing technology that can be applied to …

CRISPR/Cas9-mediated genome editing and gene replacement in plants: transitioning from lab to field

SM Schaeffer, PA Nakata - Plant Science, 2015 - Elsevier
The CRISPR/Cas9 genome engineering system has ignited and swept through the scientific
community like wildfire. Owing largely to its efficiency, specificity, and flexibility, the …

[HTML][HTML] CRISPR-Cas12a (Cpf1): a versatile tool in the plant genome editing tool box for agricultural advancement

A Bandyopadhyay, N Kancharla, VS Javalkote… - Frontiers in plant …, 2020 - frontiersin.org
Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion
from today. To meet the demands of growing, geographically and socio-economically …

Achieving plant CRISPR targeting that limits off‐target effects

JD Wolt, K Wang, D Sashital… - The plant …, 2016 - Wiley Online Library
The CRISPR‐Cas9 system (clustered regularly interspaced short palindromic repeats with
associated Cas9 protein) has been used to generate targeted changes for direct …

CRISPR-Cas9 based plant genome editing: Significance, opportunities and recent advances

N Soda, L Verma, J Giri - Plant Physiology and Biochemistry, 2018 - Elsevier
Precise genome editing is a quantum leap in the field of plant sciences. Clustered regularly
interspaced short palindromic repeats (CRISPR) and its associated Cas9 protein have …

[HTML][HTML] Gene editing and crop improvement using CRISPR-Cas9 system

L Arora, A Narula - Frontiers in plant science, 2017 - frontiersin.org
Advancements in Genome editing technologies have revolutionized the fields of functional
genomics and crop improvement. CRISPR/Cas9 (clustered regularly interspaced short …

The CRISPR–Cas system for plant genome editing: advances and opportunities

V Kumar, M Jain - Journal of experimental botany, 2015 - academic.oup.com
Genome editing is an approach in which a specific target DNA sequence of the genome is
altered by adding, removing, or replacing DNA bases. Artificially engineered hybrid …

[HTML][HTML] Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering

N Wada, R Ueta, Y Osakabe, K Osakabe - BMC Plant Biology, 2020 - Springer
Traditionally, generation of new plants with improved or desirable features has relied on
laborious and time-consuming breeding techniques. Genome-editing technologies have led …