Technological breakthroughs in generating transgene-free and genetically stable CRISPR-edited plants
CRISPR/Cas9 gene-editing technologies have been very effective in editing target genes in
all major crop plants and offer unprecedented potentials in crop improvement. A major …
all major crop plants and offer unprecedented potentials in crop improvement. A major …
The CRISPR/Cas9 system and its applications in crop genome editing
A Bao, DJ Burritt, H Chen, X Zhou, D Cao… - Critical reviews in …, 2019 - Taylor & Francis
The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR
associated protein9) system is an RNA-guided genome editing tool that consists of a Cas9 …
associated protein9) system is an RNA-guided genome editing tool that consists of a Cas9 …
Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression
L Long, DD Guo, W Gao, WW Yang, LP Hou, XN Ma… - Plant Methods, 2018 - Springer
Abstract Background When developing CRISPR/Cas9 systems for crops, it is crucial to
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …
CRISPR-Cas9: tool for qualitative and quantitative plant genome editing
Recent developments in genome editing techniques have aroused substantial excitement
among agricultural scientists. These techniques offer new opportunities for developing …
among agricultural scientists. These techniques offer new opportunities for developing …
CRISPR/Cas9 in plants: at play in the genome and at work for crop improvement
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9
(CRISPR/Cas9) system uses single-guide RNAs for genome editing, making it a simple …
(CRISPR/Cas9) system uses single-guide RNAs for genome editing, making it a simple …
Principles, applications, and biosafety of plant genome editing using CRISPR-Cas9
K El-Mounadi, ML Morales-Floriano… - Frontiers in plant …, 2020 - frontiersin.org
The terms genome engineering, genome editing, and gene editing, refer to modifications
(insertions, deletions, substitutions) in the genome of a living organism. The most widely …
(insertions, deletions, substitutions) in the genome of a living organism. The most widely …
Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
CRISPR/Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing
tools in plants. In this work, we compared their activities by targeting maize glossy2 gene …
tools in plants. In this work, we compared their activities by targeting maize glossy2 gene …
CRISPR-Cas9 editing in maize: systematic evaluation of off-target activity and its relevance in crop improvement
J Young, G Zastrow-Hayes, S Deschamps… - Scientific reports, 2019 - nature.com
CRISPR-Cas9 enabled genome engineering has great potential for improving agriculture
productivity, but the possibility of unintended off-target edits has evoked some concerns …
productivity, but the possibility of unintended off-target edits has evoked some concerns …
Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat
H Hamada, Y Liu, Y Nagira, R Miki, N Taoka, R Imai - Scientific reports, 2018 - nature.com
The current application of genome editing to crop plants is limited to cultivars that are
amenable to in vitro culture and regeneration. Here, we report an in planta genome-editing …
amenable to in vitro culture and regeneration. Here, we report an in planta genome-editing …
Challenges facing CRISPR/Cas9-based genome editing in plants
S Son, SR Park - Frontiers in plant science, 2022 - frontiersin.org
The development of plant varieties with desired traits is imperative to ensure future food
security. The revolution of genome editing technologies based on the clustered regularly …
security. The revolution of genome editing technologies based on the clustered regularly …