CRISPR-Cas9 system for plant genome editing: Current approaches and emerging developments
JAV Montecillo, LL Chu, H Bae - Agronomy, 2020 - mdpi.com
Targeted genome editing using CRISPR-Cas9 has been widely adopted as a genetic
engineering tool in various biological systems. This editing technology has been in the …
engineering tool in various biological systems. This editing technology has been in the …
CRISPR/Cas9-based gene editing using egg cell-specific promoters in Arabidopsis and soybean
CRISPR/Cas9-based systems are efficient genome editing tools in a variety of plant species
including soybean. Most of the gene edits in soybean plants are somatic and non …
including soybean. Most of the gene edits in soybean plants are somatic and non …
Efficient targeted mutagenesis in potato by the CRISPR/Cas9 system
S Wang, S Zhang, W Wang, X Xiong, F Meng, X Cui - Plant cell reports, 2015 - Springer
Technologies to achieve the specific and precise knockout of genes are critical for
understanding gene functions and fundamental biological processes. Targeted genome …
understanding gene functions and fundamental biological processes. Targeted genome …
An Effective Strategy for Reliably Isolating Heritable and Cas9-Free Arabidopsis Mutants Generated by CRISPR/Cas9-Mediated Genome Editing
X Gao, J Chen, X Dai, D Zhang, Y Zhao - Plant physiology, 2016 - academic.oup.com
Mutations generated by CRISPR/Cas9 in Arabidopsis (Arabidopsis thaliana) are often
somatic and are rarely heritable. Isolation of mutations in Cas9-free Arabidopsis plants can …
somatic and are rarely heritable. Isolation of mutations in Cas9-free Arabidopsis plants can …
Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system
K Belhaj, A Chaparro-Garcia, S Kamoun, V Nekrasov - Plant methods, 2013 - Springer
Targeted genome engineering (also known as genome editing) has emerged as an
alternative to classical plant breeding and transgenic (GMO) methods to improve crop …
alternative to classical plant breeding and transgenic (GMO) methods to improve crop …
Recent advances in genome editing using CRISPR/Cas9
Y Ding, H Li, LL Chen, K Xie - Frontiers in plant science, 2016 - frontiersin.org
The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-
associated nuclease 9) system is a versatile tool for genome engineering that uses a guide …
associated nuclease 9) system is a versatile tool for genome engineering that uses a guide …
Epigenetic features drastically impact CRISPR–Cas9 efficacy in plants
CRISPR–Cas9-mediated genome editing has been widely adopted for basic and applied
biological research in eukaryotic systems. While many studies consider DNA sequences of …
biological research in eukaryotic systems. While many studies consider DNA sequences of …
CRISPR-GE: a convenient software toolkit for CRISPR-based genome editing
Use of the clustered regularly interspaced short palindromic repeat (CRISPR)-associated
protein9 (Cas9) and Cpf1 systems in plants (Ma et al., 2016; Wang et al., 2017) involves …
protein9 (Cas9) and Cpf1 systems in plants (Ma et al., 2016; Wang et al., 2017) involves …
CRISPR/Cas9 for development of disease resistance in plants: recent progress, limitations and future prospects
S Ahmad, X Wei, Z Sheng, P Hu… - Briefings in Functional …, 2020 - academic.oup.com
Several plant pathogens severely affect crop yield and quality, thereby threatening global
food security. In order to cope with this challenge, genetic improvement of plant disease …
food security. In order to cope with this challenge, genetic improvement of plant disease …
Applications of CRISPR–Cas in agriculture and plant biotechnology
The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …
molecular biology beyond all expectations. Characterized by robustness and high target …