The revolutionary technology of CRISPR/Cas systems and their extraordinary potential to
address fundamental questions in every field of biological sciences has led to their …

Abstract Background Recently, the CRISPR/Cas9 system has been widely used to precisely
edit plant genomes. Due to the difficulty in Agrobacterium-mediated genetic transformation …

The recent development of sequence-specific nuclease systems, ie, TALENs and
CRISPR/Cas9, has made genomic targeting easier in many organisms including plants (Li …

Previous studies revealed that the promoters for driving both Cas9 and sg RNA s are quite
important for efficient genome editing by CRISPR/Cas9 in plants. Here, we report our results …

Pea (Pisum sativum L.) is an annual cool-season legume crop. Owing to its role in
sustainable agriculture as both a rotation and a cash crop, its global market is expanding …

Dear Editor, Since the beginning of CRISPR/Cas9's use for genome editing, reports of off-
targets have caused concerns among users. Previous studies identified three main types of …

Global demand for food is increasing day by day due to an increase in population and
shrinkage of the arable land area. To meet this increasing demand, there is a need to …

Main conclusion An improved CRISPR/Cas9 system with the Arabidopsis UBQ10 promoter-
driven Cas9 exhibits consistently high mutation efficiency in Arabidopsis and M. truncatula …

CRISPR/Cas mediated genome editing is a revolutionary approach for manipulating the
plant genome. However, the success of this technology is highly dependent on selection of a …

The CRISPR/Cas gene editing system offers great potential for functional genomics in plants
and crop improvement. The specificity of Cas-directed DNA cleavage is strictly determined …