The clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR
associated (Cas) 9 protein system has emerged as a simple and efficient tool for genome …

CRISPR-Cas9 systems have been widely used in animals and plants for genome editing,
epigenetic modification, etc.(Cong et al., 2013; Hilton et al., 2015). However, the accurate …

Precise and straightforward methods to edit the plant genome are much needed for
functional genomics and crop improvement. Recently, RNA-guided genome editing using …

The Cas9/sgRNA of the CRISPR/Cas system has emerged as a robust technology for
targeted gene editing in various organisms, including plants, where Cas9/sgRNA-mediated …

CRISPR-Cas9,-Cas12a,-Cas12b, and-Cas13 have been harnessed for genome
engineering in human and plant cells (Liu et al., 2022). However, the large size of these Cas …

As an allotetraploid, most genes have multiple copies that belong to At and Dt subgenomes
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n …

Since it was first recognized in bacteria and archaea as a mechanism for innate viral
immunity in the early 2010s, clustered regularly interspaced short palindromic repeats …

Cowpea (Vigna unguiculata) is widely cultivated across the world. Due to its symbiotic
nitrogen fixation capability and many agronomically important traits, such as tolerance to low …

Targeted genome editing with the CRISPR/Cas9 system has been used extensively for the
selective mutation of plant genes. Here we used CRISPR/Cas9 to disrupt the putative barley …

Common wheat supplies vast amounts of dietary carbohydrate and protein for over 60% of
world population. With continued economic development, demand for high-yielding varieties …