Cytofluorometric assay for the determination of thymidine uptake and phosphorylation in living cells
M Hengstschläuger, E Wawra - Cytometry: The Journal of the …, 1993 - Wiley Online Library
Thymidine kinase is a key enzyme for the application of drugs in chemotherapy and for
diagnosis. Although of great interest, its regulation during cell cycle and differentiation is …
diagnosis. Although of great interest, its regulation during cell cycle and differentiation is …
Cytofluorometric determination of thymidine kinase activity in a mixture of normal and neoplastic cells
M Hengstschläger, E Wawra - British journal of cancer, 1993 - nature.com
A cytofluorometric assay allowing the measurement of thymidine phosphorylation in single
cells had been established (Hengstschläger & Wawra, 1993). This assay enables us to …
cells had been established (Hengstschläger & Wawra, 1993). This assay enables us to …
Thymidine kinase expression: a marker for malignant cells
M Hengstschläger, M Pfeilstöcker, E Wawra - Purine and pyrimidine …, 1998 - Springer
The expression of thymidine kinase—an enzyme of the DNA precursor pathway—is strictly
regulated during the normal cellular cycle, but is much higher and permanently expressed in …
regulated during the normal cellular cycle, but is much higher and permanently expressed in …
Thymidine kinase is expressed differently in transformed versus normal-cells-a novel test for malignancy
M Hengstschlager, EW Mullner… - … Journal of Oncology, 1994 - spandidos-publications.com
Using a cytofluorometric assay, we studied the time course of thymidine kinase activity
during the cell-cycle in logarithmically growing cells. Two different patterns were found …
during the cell-cycle in logarithmically growing cells. Two different patterns were found …
Identification of leukaemic cells in bone marrow and blood samples by a new cytofluorometric assay
M Hengstschläger, M Pfeilstöcker, E Wawra - British journal of cancer, 1996 - nature.com
The expression of thymidine kinase--an enzyme of the DNA precursor pathway--is strictly
regulated during the normal cellular cycle, but is much higher and permanently expressed in …
regulated during the normal cellular cycle, but is much higher and permanently expressed in …
Detection of thymidine kinase activity using an assay based on the precipitation of nucleoside monophosphates with lanthanum chloride
RM Wolcott, JM Colacino - Analytical biochemistry, 1989 - Elsevier
A new assay method for the measurement of thymidine kinase (TK) is described. Cytosols
were prepared from TK− and TK+ cells and evaluated for TK activity using an assay which is …
were prepared from TK− and TK+ cells and evaluated for TK activity using an assay which is …
Microspectrophotometry and autoradiography in the study of DNA values of cells in tissue culture
CG Potter - Experimental Cell Research, 1970 - Elsevier
Tritiated thymidine labelling for various times together with Feulgen microspectrophotometry
was used to determine the presence of different cell lines in monolayer cell cultures. Using …
was used to determine the presence of different cell lines in monolayer cell cultures. Using …
Noninvasive, real-time method for the examination of thymidine uptake events—application of the method to V-79 cell synchrony studies
R Graves, R Davies, G Brophy, G O'Beirne… - Analytical biochemistry, 1997 - Elsevier
[14C] Thymidine uptake into V-79 hamster lung fibroblasts has been successfully
demonstrated using a noninvasive, real-time method utilizing Cytostar-T scintillating …
demonstrated using a noninvasive, real-time method utilizing Cytostar-T scintillating …
A stable isotope method for measurement of thymidine incorporation into DNA
HA Heck, JH McReynolds, M Anbar - Cell Proliferation, 1977 - Wiley Online Library
ABSTRACT A method has been developed for the measurement of DNA synthesis in vivo
using the incorporation of multilabeled, non‐radioactive thymidine. Simultaneous …
using the incorporation of multilabeled, non‐radioactive thymidine. Simultaneous …
Flow cytofluorometric analysis of cell cycle distributions using propidium iodide. Properties of the method and mathematical analysis of the data.
J Fried, AG Perez, BD Clarkson - The Journal of cell biology, 1976 - rupress.org
In order to better characterize the new rapid staining method for flow cytofluorometry
proposed by Krishan, we have tested its stability and several other properties, and have …
proposed by Krishan, we have tested its stability and several other properties, and have …