Publisher Summary This chapter describes the methods for sample preparation and
staining, flow-cytometric data acquisition, and the procedures for quantitative analysis of …

Thymidine kinase has been purified from E. coli B by affinity chromatography on agarose
columns to which the substrate analog 5'-amino-5'-deoxythymidine (= 5'-amino-2', 5' …

Thymidine kinase from a transplantable colon adenocarcinoma, induced by 1, 2-
dimethylhydrazine and maintained in CDF rats, was purified by affinity chromatography …

The direct assay of thymidine kinase (Tk) bound to ion-exchange paper was investigated as
a means to further simplify the analytical procedure. Thymidine kinase bound firmly and …

In mammalian proliferating cells cytosolic thymidine kinase (TK1) phosphorylate thymidine
and deoxyuridine to the corresponding monophosphate, thus providing precursors for DNA …

Activity levels of thymidine kinase and thymidylate kinase were determined in extracts from
23 matched human neoplastic and normal tissue pairs. Thymidine kinase activity was higher …

This report describes the regulation of thymidine kinase (TK) enzyme level in mouse 3T6
fibroblasts experiencing a serum-induced transition between the resting (G0) and growing …

A continuous spectrophotometric assay of deoxythymidine (dThd) phosphorylase is reported
which monitors conversion of 5-nitro-2′-deoxyuridine to 5-nitrouracil. The reaction is …

Among normal adult tissues, bone marrow, thymus, small intestine and spleen had
significant thymidine kinase activity (61, 16.4, 4.0 and 3.8 units/g); in kidney, brain, lung …

Measurement of a DNA histogram can be achieved by fixing or permeabilizing cells and
staining them with a DNA-binding dye, such as propidium iodide (PI). The histogram will …