The possibility was investigated that thymidine phosphorylase activity is released from
leukocytes during ultrafiltration of crude “plasma,” thus accounting for reports of this enzyme …

Measurement of a DNA histogram can be achieved by fixing or permeabilizing cells and
staining them with a DNA-binding dye, such as propidium iodide (PI). The histogram will …

We report the development and characterization of a semiautomated method for
measurement of cell proliferation in microculture using Hoechst 33342, a non-toxic specific …

The expression of different variants of thymidine kinase (TdR kinase), characterized by
electrophoreUc mobilities, is related to the replicative state of normal or transformed cultured …

For young investigators who presently enter their scientific pursuit focused on cell cycle and
have flow cytometry (FCM) at their disposal, it may be difficult to visualize the hardship of …

Results are presented supporting the study of cellular proliferation utilizing 5-
bromodeoxyuridine (BrdU) incorporation followed by sister chromatid differential staining. In …

Thymidine phosphorylase levels are higher in some human cancer tissues than in adjacent
normal tissue. However, the ultrastructural localization of thymidine phosphorylase in cancer …

The mammalian cytosolic thymidine kinase is one of a number of enzymes involved in DNA
replication whose activities increase dramatically during S phase of the cell cycle. As a first …

The dispersal of solid tissues into a single-cell suspension has become an integral
component of many current techniques for quantitative cytokinetic analysis of both normal …

A method is described to combine tritiated thymidine autoradiography with
immunoperoxidase detection of bromodeoxyuridine on the same paraffin sections. It …