Since the inception of modern embryo culture media over 50 years ago there have been
significant developments in culture systems for the mammalian preimplantation embryo …

The first culture media designed specifically to support development of the preimplantation
mouse embryo were formulated over 50 years ago and were based on balanced salt …

Considerable advances in media development for the culture of preimplantation mammalian
embryos have been made since mouse embryos were first cultured and successfully …

There are several treatises on the composition of embryo culture media and the role of
specific medium components in supporting mammalian embryo development in vitro (1–3). It …

Several media, some augmented with amino acids, have been formulated recently, based
on simplex optimization, to support the preimplantation development of mouse embryos. For …

Methods for the culture of preimplantation human embryos evolved primarily from those
used for mouse embryos. The initial method was usually culture in a single medium in …

We have recently reported the use of sequential simplex optimization methods to design a
medium (SOM) that overcomes the block to development beyond two cells which occurs in …

Publisher Summary During the preimplantation period of mammalian embryo development,
the conceptus undergoes significant changes in its physiology, metabolism, and genetic …

A limiting factor in the development of new technologies and transport of rats worldwide has
been the inability to robustly culture preimplantation embryos. Previously, culture in vitro to …

The field of modern embryo culture spans several decades. However, in the last decade of
the 20th century a resurgence of interest in embryo physiology and metabolism led to highly …