Cryptococcus neoformans is an opportunistic fungal pathogen that primarily affects
immunocompromised individuals. Reverse genetics is commonly used to identify and …

Reverse genetics is commonly used to identify and characterize genes involved in a variety
of cellular processes. There is a limited set of positive selectable markers available for use in …

Cryptococcus neoformans is an opportunistic fungal pathogen with a defined sexual cycle
for which genetic and molecular techniques are well developed. The entire genome …

While the fungal pathogen Cryptoccocus neoformans is a leading cause of death in
immunocompromised individuals, the molecular toolkit currently available to study this …

Antisense repression was used as a method to alter gene function in the human-pathogenic
fungus Cryptococcus neoformans. The calcineurin A gene (CNA1) and the laccase gene …

Gene disruption by biolistic transformation in serotype D strains of Cryptococcus
neoformans. Fungal Genetics and Biology29, 38–48. Cryptococcus neoformans is an …

Cryptococcus neoformans and Cryptococcus gattii are basidiomycetous fungi that infect
immunocompromised and immunocompetent people. We developed an insertional …

▪ Abstract Cryptococcus neoformans is a pathogenic fungus that primarily afflicts
immunocompromised patients, infecting the central nervous system to cause …

Gene disruption in Cryptococcus neoformans can be problematic due to high frequencies of
ectopic integration and telomerization. To improve the frequency of homologous integration …

Cryptococcus neoformans is an opportunistic human fungal pathogen and serves as a
model organism for studies of eukaryotic microbiology and microbial pathogenesis. C …