Effects of amino acids on early bovine embryo development in vitro were examined using a
chemically-defined, protein-free culture medium. Bovine embryos produced in vitro were …

The aim of the study was to determine the amino acid requirements of the in vitro-produced
bovine embryo as it develops from the zygote to the blastocyst, using a two-step culture …

Amino acids have been shown to improve the development of in vitro fertilised bovine 1-cell
embryos to morulae and blastocysts, and increase total cell numbers at the blastocyst stage …

The effect of glycine and alanine on the development of 2-to 4-cell bovine embryos, and
amino acid uptake by bovine morulae and blastocysts, were examined through the use of a …

Due to the complicated media used for culturing bovine embryos, most of the nutrient
requirements are unknown. Recently, we developed a simple, serum-free medium (CR1) …

In experiment 1, the effects of a group of either 20 (ie glutamine+ essential+ non-essential)
or 11 (ie hamster embryo culture medium (HECM)-6) amino acids were evaluated in …

This study was conducted to elucidate the role of amino acids added singly or in groups to a
chemically defined culture medium in blastocyst formation and blastomere proliferation of …

Bovine embryos produced in vitro from the putative zygote stage to the blastocyst stage, and
blastocysts freshly flushed from the uterus, were cultured in a physiological mixture of amino …

Five experiments, utilizing 3741 embryos produced in vitro, were designed to test the effects
of Eagle's nonessential amino acids, and combinations of Eagle's essential amino acids and …

Two major considerations governing choice of culture system for in vitro production of
bovine and other mammalian embryos are the efficiency of blastocyst production and the …