Highlights•We review high-throughput mass spectrometry-based methods for metabolomic
analysis.•High-throughput approaches usually improve throughput at the expense of …

In order to perform metabonomic investigations, it is necessary to generate comprehensive
“global” metabolite profiles from complex samples, such as biofluids and tissues/tissue …

Recent progress in metabolomics and the development of increasingly sensitive analytical
techniques have renewed interest in global profiling, ie, semiquantitative monitoring of all …

Mass spectrometry-based metabolomics approaches can enable detection and
quantification of many thousands of metabolite features simultaneously. However …

The metabolic composition of human biofluids can provide important diagnostic and
prognostic information. Among the biofluids most commonly analyzed in metabolomic …

The choice of sample-preparation method is extremely important in metabolomic studies
because it affects both the observed metabolite content and biological interpretation of the …

The effect of diet on metabolites found in rat urine samples has been investigated using
nuclear magnetic resonance (NMR) and a new ambient ionization mass spectrometry …

Metabolic fingerprinting, the main tool in metabolomics, is a non-targeted methodology
where all detectable peaks (or signals), including those from unknown analytes, are …

Properly implemented, metabolic and lipidomic profiling can provide a deeper
understanding of mammalian, plant and bacterial biology. These omics-tools have …

Metabonomics and metabolomics represent one of the three major platforms in systems
biology. To perform metabolomics it is necessary to generate comprehensive “global” …