Optimization of the SARS-CoV-2 ARTIC network V4 primers and whole genome sequencing protocol

AW Lambisia, KS Mohammed, TO Makori… - Frontiers in …, 2022 - frontiersin.org
Introduction The ARTIC Network's primer set and amplicon-based protocol is one of the most
widely used SARS-CoV-2 sequencing protocol. An update to the V3 primer set was released …

[HTML][HTML] Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing using nanopore

JR Tyson, P James, D Stoddart, N Sparks… - BioRxiv, 2020 - ncbi.nlm.nih.gov
Genome sequencing has been widely deployed to study the evolution of SARS-CoV-2 with
more than 90,000 genome sequences uploaded to the GISAID database. We published a …

Sensitive recovery of complete SARS-CoV-2 genomes from clinical samples by use of Swift Biosciences' SARS-CoV-2 multiplex amplicon sequencing panel

A Addetia, MJ Lin, V Peddu… - Journal of clinical …, 2020 - Am Soc Microbiol
Whole-genome sequencing (WGS) of severe acute respiratory syndrome coronavi-rus 2
(SARS-CoV-2) has led to a better understanding of the virus's origin, transmission, and …

Whole-genome sequencing of sars-cov-2: Assessment of the ion torrent ampliseq panel and comparison with the illumina miseq artic protocol

J Plitnick, S Griesemer… - Journal of Clinical …, 2021 - Am Soc Microbiol
Fast and effective methods are needed for sequencing of the severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) genome to track genetic mutations and to identify …

CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes

DJ Baker, A Aydin, T Le-Viet, GL Kay, S Rudder… - Genome medicine, 2021 - Springer
We present CoronaHiT, a platform and throughput flexible method for sequencing SARS-
CoV-2 genomes (≤ 96 on MinION or> 96 on Illumina NextSeq) depending on changing …

[HTML][HTML] nCoV-2019 sequencing protocol v3 (LoCost)

J Quick - 2020 - slack.protocols.io
Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing
using nanopore. John R Tyson, et. al. bioRxiv 2020.09. 04.283077; doi: https://doi …

A comparison of whole genome sequencing of SARS-CoV-2 using amplicon-based sequencing, random hexamers, and bait capture

JA Nasir, RA Kozak, P Aftanas, AR Raphenya… - Viruses, 2020 - mdpi.com
Genome sequencing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is
increasingly important to monitor the transmission and adaptive evolution of the virus. The …

Disentangling primer interactions improves SARS-CoV-2 genome sequencing by multiplex tiling PCR

K Itokawa, T Sekizuka, M Hashino, R Tanaka… - PloS one, 2020 - journals.plos.org
Since December 2019, the coronavirus disease 2019 (COVID-19) caused by a novel
coronavirus SARS-CoV-2 has rapidly spread to almost every nation in the world. Soon after …

Assessment of SARS-CoV-2 genome sequencing: quality criteria and low-frequency variants

D Jacot, T Pillonel, G Greub… - Journal of Clinical …, 2021 - Am Soc Microbiol
Although many laboratories worldwide have developed their sequencing capacities in
response to the need for SARS-CoV-2 genome-based surveillance of variants, only a few …

Multiple approaches for massively parallel sequencing of SARS-CoV-2 genomes directly from clinical samples

M Xiao, X Liu, J Ji, M Li, J Li, L Yang, W Sun, P Ren… - Genome medicine, 2020 - Springer
Abstract Background COVID-19 (coronavirus disease 2019) has caused a major epidemic
worldwide; however, much is yet to be known about the epidemiology and evolution of the …