This protocol is an update from the protocol described in the paper" Rapid and sensitive
direct detection and identification of poliovirus from stool and environmental surveillance …

This protocol is an update from the protocol described in the paper" Rapid and sensitive
direct detection and identification of poliovirus from stool and environmental surveillance …

This protocol is updated from the protocol described in the paper" Rapid and sensitive direct
detection and identification of poliovirus from stool and environmental surveillance samples …

Global poliovirus surveillance involves virus isolation from stool and environmental samples,
intratypic differential (ITD) by PCR, and sequencing of the VP1 region to distinguish vaccine …

Timely detection of outbreaks is needed for poliovirus eradication, but gold standard
detection in the Democratic Republic of the Congo takes 30 days (median). Direct molecular …

Delayed detection of poliovirus outbreaks is a major threat to polio eradication. Direct
molecular Detection and Nanopore Sequencing (DDNS) of stool samples shows promise as …

This short FAQ document summarises the poliovirus direct detection and nanopore
sequencing (DDNS) protocol, the equipment and reagents required, the cost and staff-time …

This document describes the laboratory process for the use of run controls (positive and
negative controls) for performing direct detection of poliovirus by nanopore sequencing …

Widespread surveillance, rapid detection, and appropriate intervention will be critical for
successful eradication of poliovirus. Using deployable next-generation sequencing (NGS) …

Direct detection by PCR of poliovirus RNA in stool samples provides a rapid diagnostic and
surveillance tool that can replace virus isolation by cell culture in global polio surveillance …