The present article describes a genome database reviewing gene-related knowledge of two
model bacteria, Bacillus subtilis and Escherichia coli. The database, Indigo, is open through …

SPP1 DNA was cleaved by the restriction endonucleases, Bgl I, Bgl II, Eco RI, Kpn I, Sma I,
and Sal I. The molecular weights of the DNA fragments obtained by single enzyme digestion …

The aim of this chapter is to summarize the commercial reasons for developing expression
systems for making recombinant products in B. subtilis, to describe the problems involved in …

Bacillus subtilis∆ 6 is a genome-reduced strain that was cured from six prophages and AT-
rich islands. This strain is of great interest for biotechnological applications. Here, we …

An expression vector for systematic protein overproduction in Bacillus subtilis has been
constructed. It derives from pDG148 and combines the main property of this vector, ie …

We have determined a 180 kb contiguous sequence in the replication origin region of the
Bacillus subtilis chromosome. Open reading frames (ORF) in this region were …

ABSTRACT Next to Escherichia coli, Bacillus subtilis is the most studied and best
understood organism that also serves as a model for many important pathogens. Due to its …

To study the functions of the uncharacterized open reading frames identified in the Bacillus
subtih genome, several vectors were constructed to perform insertional mutagenesis in the …

Cloning in Escherichia coli and Bacillus subtilis was carried out using the bifunctional
plasmid pDH5060. B. subtilis chromosomal DNA and pDH5060 DNA were digested with …

Extracellular Bacillus proteases are used as additives in detergent powders. We identified a
Bacillus strain that produces a protease with an extremely alkaline pH optimum; this …