Development of Agrobacterium-Mediated Virus-Induced Gene Silencing and Performance Evaluation of Four Marker Genes in Gossypium barbadense

J Pang, Y Zhu, Q Li, J Liu, Y Tian, Y Liu, J Wu - PloS one, 2013 - journals.plos.org
Gossypium barbadense is a cultivated cotton species and possesses many desirable traits,
including high fiber quality and resistance to pathogens, especially Verticillium dahliae (a …

Virus-Induced Gene Silencing in Cultivated Cotton (Gossypium spp.) Using Tobacco Rattle Virus

R Mustafa, M Shafiq, S Mansoor, RW Briddon… - Molecular …, 2016 - Springer
The study described here has optimized the conditions for virus-induced gene silencing
(VIGS) in three cultivated cotton species (Gossypium hirsutum, G. arboreum, and G …

Functional genomic analysis of cotton genes with agrobacterium-mediated virus-induced gene silencing

X Gao, L Shan - Virus-Induced Gene Silencing: Methods and Protocols, 2013 - Springer
Cotton (Gossypium spp.) is one of the most agronomically important crops worldwide for its
unique textile fiber production and serving as food and feed stock. Molecular breeding and …

Agrobacterium-mediated virus-induced gene silencing assay in cotton

X Gao, RC Britt Jr, L Shan, P He - JoVE (Journal of Visualized …, 2011 - jove.com
Cotton (Gossypium hirsutum) is one of the most important crops worldwide. Considerable
efforts have been made on molecular breeding of new varieties. The large-scale gene …

Silencing GhNDR1 and GhMKK2 compromises cotton resistance to Verticillium wilt

X Gao, T Wheeler, Z Li, CM Kenerley, P He… - The Plant …, 2011 - Wiley Online Library
Cotton is an important cash crop worldwide, and is a significant source of fiber, feed,
foodstuff, oil and biofuel products. Considerable effort has been expended to increase …

Host‐induced gene silencing of a regulator of G protein signalling gene (VdRGS1) confers resistance to Verticillium wilt in cotton

J Xu, X Wang, Y Li, J Zeng, G Wang… - Plant biotechnology …, 2018 - Wiley Online Library
Verticillium wilt (VW), caused by soil‐borne fungi of the genus Verticillium, is a serious
disease affecting a wide range of plants and leading to a constant and major challenge to …

Generation of transcriptome profiling and gene functional analysis in Gossypium hirsutum upon Verticillium dahliae infection

W Zhang, H Zhang, F Qi, G Jian - Biochemical and Biophysical Research …, 2016 - Elsevier
Verticillium wilt caused by the soil-borne fungus Verticillium dahliae, is a devastating
disease in cotton. To get more candidate genes related to wilt resistance, a normalized, full …

A novel VIGS method by agroinoculation of cotton seeds and application for elucidating functions of GhBI-1 in salt-stress response

J Zhang, F Wang, C Zhang, J Zhang, Y Chen, G Liu… - Plant cell reports, 2018 - Springer
Key message A VIGS method by agroinoculation of cotton seeds was developed for gene
silencing in young seedlings and roots, and applied in functional analysis of GhBI-1 in …

A versatile system for functional analysis of genes and micro RNA s in cotton

Z Gu, C Huang, F Li, X Zhou - Plant biotechnology journal, 2014 - Wiley Online Library
Cotton is an important economic crop worldwide. Due to its long growth cycle, large genome
size and recalcitrance to stable transformation, traditional methods for the analysis of gene …

Rationale for developing new virus vectors to analyze gene function in grasses through virus-induced gene silencing

H Ramanna, XS Ding, RS Nelson - Virus-induced gene silencing …, 2013 - Springer
The exploding availability of genome and EST-based sequences from grasses requires a
technology that allows rapid functional analysis of the multitude of genes that these …