Virus‐induced gene silencing (VIGS) is an extremely powerful tool for plant functional
genomics. We used Tobacco rattle virus (TRV)‐derived VIGS vectors expressed from binary …

Background We previously developed a virus-induced gene silencing (VIGS) vector for
cotton from the bipartite geminivirus Cotton leaf crumple virus (CLCrV). The original CLCrV …

Key message Target genes in rice can be optimally silenced if inserted in antisense or
hairpin orientation in the RTBV-derived VIGS vector and plants grown at 28° C and 80 …

Agrobacterium-mediated transient expression and virus-induced gene silencing (VIGS) are
very useful in functional genomics in plants. However, whether these methods are effective …

Virus‐induced gene silencing (VIGS) is currently recognized as a powerful reverse genetics
tool for application in functional genomics. DNA1, a satellite‐like and single‐stranded DNA …

Aim We report the construction of a Virus-Induced Gene Silencing (VIGS) vector and an
agroinoculation protocol for gene silencing in cassava (Manihot esculenta Crantz) leaves …

A silencing vector for cotton (Gossypium hirsutum) was developed from the geminivirus
Cotton leaf crumple virus (CLCrV). The CLCrV coat protein gene was replaced by up to 500 …

Background Although the draft genome of sorghum is available, the understanding of gene
function is limited due to the lack of extensive mutant resources. Virus-induced gene …

Virus-induced gene silencing (VIGS) is a recently developed technique for characterizing
the function of plant genes by gene transcript suppression and is increasingly used to …

Verticillium wilt of cotton (Gossypium hirsutum) is a widespread and destructive disease that
is caused by the soil-borne fungus pathogen Verticillium dahliae (V. dahliae). To study the …