Following the introduction of fluorescent protein tags, the application of fluorescence
microscopy in microbial cell biology has advanced the field dramatically. We now …

Fluorescence-imaging techniques are now being applied to single bacterial cells for the
determination of in vivo kinase and transcription activity, protein–protein interactions, protein …

Recent developments on fluorescent proteins and microscopy techniques have allowed the
probing of single molecules in a living bacterial cell with high specificity, millisecond time …

In addition to the inherent interest stemming from their ecological and human health impacts,
microbes have many advantages as model organisms, including ease of growth and …

Highlights•Bacterial cell biology has powerful synergies with systems biology.•Single-cell
and single-molecule imaging reveal the robustness of cell-wall growth.•Genetic pathways …

Fluorescent proteins and developments in superresolution (nanoscopy) and single-
molecule techniques bring high sensitivity, speed, and one order of magnitude gain in …

Core cellular processes such as DNA replication and segregation, protein synthesis, cell
wall biosynthesis, and cell division rely on the function of proteins which are essential for …

Advances in microscopy and fluorescent probes provide new insight into the nanometer‐
scale biochemistry governing the interactions between eukaryotic cells and pathogens …

Bacteria are microorganisms central to health and disease, serving as important model
systems for our understanding of molecular mechanisms and for developing new …

Every living organism—including Earth's simplest life form, the bacterium—is loaded with
molecular devices that are breathtaking in their design, complexity, and efficiency …