Comprehensive detection and serotyping of human adenoviruses by PCR and sequencing

H Sarantis, G Johnson, M Brown… - Journal of Clinical …, 2004 - Am Soc Microbiol
Human adenoviruses are common pathogens associated with many diseases, including
respiratory, gastrointestinal, and ocular infections. Because they are now being increasingly …

Molecular detection and quantitative analysis of the entire spectrum of human adenoviruses by a two-reaction real-time PCR assay

K Ebner, M Suda, F Watzinger… - Journal of Clinical …, 2005 - Am Soc Microbiol
Adenoviruses (AdV) can cause life-threatening infections in immunosuppressed patients.
Reliable diagnostic tests are therefore of paramount importance. Apparently, any of the six …

Rapid identification of human adenovirus types 3 and 7 from respiratory specimens via multiplex type-specific PCR

JA Lee, NH Kim, SJ Kim, EH Choi… - Journal of Clinical …, 2005 - Am Soc Microbiol
The rapid diagnosis of human adenovirus (Ad) infection is crucial for the timely recognition
of epidemics. Moreover, identification of the serotypes known to cause serious disease can …

Multiplex polymerase chain reaction for subgenus‐specific detection of human adenoviruses in clinical samples

P Pring‐Åkerblom, FE John Trijssenaar… - Journal of medical …, 1999 - Wiley Online Library
The polymerase chain reaction (PCR) has been used previously for the detection and typing
of adenoviruses directly in clinical samples. Since under clinical conditions subgenus …

Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region

I Casas, A Avellón, M Mosquera… - Journal of clinical …, 2005 - Am Soc Microbiol
Here we present a system for adenovirus detection and genotyping based on PCR
amplification and phylogenetic analysis of a conserved hexon gene fragment. The system …

Multiplexed, real-time PCR for quantitative detection of human adenovirus

Z Gu, SW Belzer, CS Gibson… - Journal of clinical …, 2003 - Am Soc Microbiol
Adenovirus infection is becoming increasingly recognized as a cause of morbidity and
mortality in the immunosuppressed patient population. While early detection and …

PCR and restriction endonuclease analysis for rapid identification of human adenovirus subgenera

EM Elnifro, RJ Cooper, PE Klapper… - Journal of Clinical …, 2000 - Am Soc Microbiol
Subgenus identification of adenoviruses is of clinical importance and is as informative as
identification by serotype in most clinical situations. A PCR-based identification of …

Rapid detection and identification of human adenovirus species by adenoplex, a multiplex PCR-enzyme hybridization assay

K Pehler-Harrington, M Khanna… - Journal of clinical …, 2004 - Am Soc Microbiol
Human adenoviruses (AdV) have been implicated in a wide variety of diseases and are
ubiquitous in populations worldwide. These agents are of concern particularly in …

Quantitative real-time PCR assays for detection of human adenoviruses and identification of serotypes 40 and 41

N Jothikumar, TL Cromeans, VR Hill, X Lu… - Applied and …, 2005 - Am Soc Microbiol
ABSTRACT A quantitative real-time TaqMan PCR assay for detection of human
adenoviruses (HAdV) was developed using broadly reactive consensus primers and a …

Real-time PCR with an internal control for detection of all known human adenovirus serotypes

M Damen, R Minnaar, P Glasius… - Journal of clinical …, 2008 - Am Soc Microbiol
The “gold standard” for the diagnosis of adenovirus (AV) infection is virus culture, which is
rather time-consuming. Especially for immunocompromised patients, in whom severe …