Targeted mutagenesis in cotton (Gossypium hirsutum L.) using the CRISPR/Cas9 system
X Chen, X Lu, N Shu, S Wang, J Wang, D Wang… - Scientific reports, 2017 - nature.com
Abstract The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9
system has been widely used for genome editing in various plants because of its simplicity …
system has been widely used for genome editing in various plants because of its simplicity …
CRISPR/Cas9-mediated targeted mutagenesis in upland cotton (Gossypium hirsutum L.)
MR Janga, LAM Campbell, KS Rathore - Plant molecular biology, 2017 - Springer
The clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR
associated (Cas) 9 protein system has emerged as a simple and efficient tool for genome …
associated (Cas) 9 protein system has emerged as a simple and efficient tool for genome …
Genome editing in cotton with the CRISPR/Cas9 system
Genome editing is an important tool for gene functional studies as well as crop improvement.
The recent development of the CRISPR/Cas9 system using single guide RNA molecules …
The recent development of the CRISPR/Cas9 system using single guide RNA molecules …
A high-efficiency CRISPR/Cas9 system for targeted mutagenesis in Cotton (Gossypium hirsutum L.)
The complex allotetraploid genome is one of major challenges in cotton for repressing gene
expression. Developing site-specific DNA mutation is the long-term dream for cotton …
expression. Developing site-specific DNA mutation is the long-term dream for cotton …
Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression
L Long, DD Guo, W Gao, WW Yang, LP Hou, XN Ma… - Plant Methods, 2018 - Springer
Abstract Background When developing CRISPR/Cas9 systems for crops, it is crucial to
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …
[HTML][HTML] Robust CRISPR/Cpf1 (Cas12a)‐mediated genome editing in allotetraploid cotton (Gossypium hirsutum)
B Li, H Rui, Y Li, Q Wang, M Alariqi, L Qin… - Plant biotechnology …, 2019 - ncbi.nlm.nih.gov
As an allotetraploid, most genes have multiple copies that belong to At and Dt subgenomes
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n …
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n …
CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum
J Gao, G Wang, S Ma, X Xie, X Wu, X Zhang, Y Wu… - Plant molecular …, 2015 - Springer
Genome editing is one of the most powerful tools for revealing gene function and improving
crop plants. Recently, RNA-guided genome editing using the type II clustered regularly …
crop plants. Recently, RNA-guided genome editing using the type II clustered regularly …
RNA-guided genome editing for target gene mutations in wheat
The clustered, regularly interspaced, short palindromic repeats (CRISPR) and CRISPR-
associated protein (Cas) system has been used as an efficient tool for genome editing. We …
associated protein (Cas) system has been used as an efficient tool for genome editing. We …
Targeted mutagenesis using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in common wheat
S Zhang, R Zhang, G Song, J Gao, W Li, X Han… - BMC plant biology, 2018 - Springer
Abstract Background Recently, the CRISPR/Cas9 system has been widely used to precisely
edit plant genomes. Due to the difficulty in Agrobacterium-mediated genetic transformation …
edit plant genomes. Due to the difficulty in Agrobacterium-mediated genetic transformation …
The application of a heat‐inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton (G. hirsutum) plants
Q Wang, M Alariqi, F Wang, B Li, X Ding… - Plant biotechnology …, 2020 - Wiley Online Library
Summary The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for
genome editing. A new effector with a single nuclease domain, a relatively small size, low …
genome editing. A new effector with a single nuclease domain, a relatively small size, low …
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