Virus-like particles (VLPs) are of interest in vaccination, gene therapy and drug delivery, but
their potential has yet to be fully realized. This is because existing laboratory processes …

Transfection of surface adherent cells remain as a standard methodology for lentiviral
production for early phase clinical studies and research purposes. Production today is …

The production of viral vaccines in cell culture can be accomplished with primary, diploid, or
continuous (transformed) cell lines. Each cell line, each virus type, and each vaccine …

Abstract Background Lentiviral vectors (LVVs) hold great promise as delivery tools for gene
therapy and chimeric antigen receptor T cell (CAR‐T) therapy. Their ability to target difficult …

We established a novel cell‐free protein synthesis system derived from Trichoplusia ni
(HighFive) insect cells by a simple extraction method. Luciferase and β‐galactosidase were …

This article describes a novel method merging the cloning of viral vector producer cells with
vector titer screening, allowing for screening 200–500 clones in 2 weeks. It makes use of a …

Baculovirus expression systems for recombinant proteins have found widespread use
because they produce biologically active proteins more reliably than do bacterial or yeast …

Insect cells (IC) and particularly lepidopteran cells are an attractive alternative to mammalian
cells for biomanufacturing. Insect cell culture, coupled with the lytic expression capacity of …

In an attempt to improve the overall efficiency of the production of heterologous proteins by
Baculovirus infected insect cell culture, we developed a perfusion bioreactor system with a …

ABSTRACT The Insect Cell-Baculovirus Expression Vector System (IC-BEVS) is widely used
for the production of complex recombinant (glyco) proteins. The simplicity of insect cell …