Identification and Discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by Real-Time PCR Targeting Type III Secretion System Genes
FM Thibault, E Valade, DR Vidal - Journal of clinical microbiology, 2004 - Am Soc Microbiol
Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for
melioidosis and glanders, respectively. The two are closely related and can also be …
melioidosis and glanders, respectively. The two are closely related and can also be …
Development and Evaluation of a Real-Time PCR Assay Targeting the Type III Secretion System of Burkholderia pseudomallei
RT Novak, MB Glass, JE Gee, D Gal… - Journal of clinical …, 2006 - Am Soc Microbiol
Here we report on the development of a discriminatory real-time assay for the rapid
identification of Burkholderia pseudomallei isolates and the evaluation of this assay for …
identification of Burkholderia pseudomallei isolates and the evaluation of this assay for …
Single gene target bacterial identification: groEL gene sequencing for discriminating clinical isolates of Burkholderia pseudomallei and Burkholderia thailandensis
PCY Woo, GKS Woo, SKP Lau, SSY Wong… - … and infectious disease, 2002 - Elsevier
Proper identification of Burkholderia pseudomallei and Burkholderia thailandensis is crucial
in guiding clinical management of patients with suspected melioidosis, as more than 99% of …
in guiding clinical management of patients with suspected melioidosis, as more than 99% of …
Laboratory diagnosis of melioidosis: past, present and future
Melioidosis is an emerging, potentially fatal disease caused by Burkholderia pseudomallei,
which requires prolonged antibiotic treatment to prevent disease relapse. However …
which requires prolonged antibiotic treatment to prevent disease relapse. However …
Detection and differentiation of Burkholderia pseudomallei, Burkholderia mallei and Burkholderia thailandensis by multiplex PCR
MA Lee, D Wang, EH Yap - FEMS Immunology & Medical …, 2005 - academic.oup.com
Burkholderia pseudomallei, a Gram-negative bacterium that causes melioidosis may be
differentiated from closely related species of Burkholderia mallei that causes glanders and …
differentiated from closely related species of Burkholderia mallei that causes glanders and …
Rapid presumptive identification of Burkholderia pseudomallei with real-time PCR assays using fluorescent hybridization probes
H Tomaso, TL Pitt, O Landt, S Al Dahouk… - Molecular and cellular …, 2005 - Elsevier
Burkholderia pseudomallei (the etiologic agent of melioidosis) can cause pyogenic or
granulomatous lesions in almost any organ. Septicemia has a case fatality rate of> 40 …
granulomatous lesions in almost any organ. Septicemia has a case fatality rate of> 40 …
Cloning and analysis of genomic differences unique to Burkholderia pseudomallei by comparison with B. thailandensis
NF Brown, IR Beacham - Journal of medical microbiology, 2000 - microbiologyresearch.org
Melioidosis is an infectious disease caused by Burkholderia pseudomallei. Genomic
subtractive hybridisation was performed with the closely related avirulent species B …
subtractive hybridisation was performed with the closely related avirulent species B …
Whole-genome sequencing of Burkholderia pseudomallei isolates from an unusual melioidosis case identifies a polyclonal infection with the same multilocus …
Twelve Burkholderia pseudomallei isolates collected over a 32-month period from a patient
with chronic melioidosis demonstrated identical multilocus sequence types (STs). However …
with chronic melioidosis demonstrated identical multilocus sequence types (STs). However …
Use of 16S rRNA Gene Sequencing for Rapid Identification and Differentiation of Burkholderia pseudomallei and B. mallei
JE Gee, CT Sacchi, MB Glass, BK De… - Journal of clinical …, 2003 - Am Soc Microbiol
Burkholderia pseudomallei and B. mallei, the causative agents of melioidosis and glanders,
respectively, are designated category B biothreat agents. Current methods for identifying …
respectively, are designated category B biothreat agents. Current methods for identifying …
Development of Real-Time PCR Assays and Evaluation of Their Potential Use for Rapid Detection of Burkholderia pseudomallei in Clinical Blood Specimens
C Supaprom, D Wang, C Leelayuwat… - Journal of clinical …, 2007 - Am Soc Microbiol
The early initiation of appropriate antimicrobial therapy is critical for improving the prognosis
of patients with septicemic melioidosis. Thus, the use of a rapid molecular diagnosis may …
of patients with septicemic melioidosis. Thus, the use of a rapid molecular diagnosis may …
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