Experiments to improve in vitro fertilization techniques for in vivo-matured porcine oocytes
D Rath - Theriogenology, 1992 - Elsevier
Three experiments were designed for the in vitro fertilization of in vivo matured oocytes
following different sperm treatments: In experiment I, spermatozoa (sperm rich fraction) were …
following different sperm treatments: In experiment I, spermatozoa (sperm rich fraction) were …
Maturation, fertilization and complete development of porcine oocytes matured under different systems
This study was designed 1) to determine the effectiveness of 2 in vitro maturation systems
commonly employed to produce nuclear and cytoplasmicly mature pig oocytes, 2) to assess …
commonly employed to produce nuclear and cytoplasmicly mature pig oocytes, 2) to assess …
Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified tris-buffered medium with frozen-thawed ejaculated spermatozoa
LR Abeydeera, BN Day - Biology of reproduction, 1997 - academic.oup.com
The present study examined the penetrability of pig oocytes by frozen-thawed ejaculated
boar spermatozoa, prepared by the pellet method, coincubated in a modified Tris-buffered …
boar spermatozoa, prepared by the pellet method, coincubated in a modified Tris-buffered …
Development potential of bovine oocytes matured in vitro or in vivo
ML Leibfried-Rutledge, ES Critser… - Biology of …, 1987 - academic.oup.com
Bovine oocytes matured in vivo or in vitro were evaluated after sperm-oocyte incubation for
frequency of sperm penetration, frequency of male pronuclei formation, and embryonic …
frequency of sperm penetration, frequency of male pronuclei formation, and embryonic …
In vitro fertilization of in vitro matured pig oocytes: effects of boar and ejaculate fraction
X Xu, J Ding, PC Seth, DS Harbison, GR Foxcroft - Theriogenology, 1996 - Elsevier
Ejaculates from 3 young boars were collected on 4 occasions as a series of separate 15-ml
fractions. The contribution of different fractions of these ejaculates to observed variability in …
fractions. The contribution of different fractions of these ejaculates to observed variability in …
Effect of sperm exposure time on in vitro fertilization and embryo development of bovine oocytes matured in vitro
N Rehman, AR Collins, TK Suh, RW Wright Jr - Theriogenology, 1994 - Elsevier
This study was designed to investigate the effect of sperm exposure time on the fertilization
rate and subsequent developmental capacity of bovine oocytes matured in vitro. Cumulus …
rate and subsequent developmental capacity of bovine oocytes matured in vitro. Cumulus …
In vitro maturation of porcine oocytes using a defined medium and developmental capacity after intracytoplasmic sperm injection
R Kishida, ES Lee, Y Fukui - Theriogenology, 2004 - Elsevier
To establish a defined in vitro maturation culture system for porcine oocytes, we examined
the effects of adding cysteine (Cys) and epidermal growth factor (EGF) to the maturation …
the effects of adding cysteine (Cys) and epidermal growth factor (EGF) to the maturation …
Brief coincubation of gametes in porcine in vitro fertilization: role of sperm: oocyte ratio and post-coincubation medium
In this study, a short coincubation time of 10min was used to determine the effect of different
sperm: oocyte ratios during in vitro fertilization (IVF), and different periods of post …
sperm: oocyte ratios during in vitro fertilization (IVF), and different periods of post …
Development of in vivo-matured porcine oocytes following intracytoplasmic sperm injection
MJ Martin - Biology of Reproduction, 2000 - academic.oup.com
The objective of this study was to assess the development of porcine ova fertilized by
intracytoplasmic sperm injection (ICSI). Allyl trenbolone (Regumate) was used to …
intracytoplasmic sperm injection (ICSI). Allyl trenbolone (Regumate) was used to …
In-vitro fertilization of pig oocytes by frozen boar spermatozoa
T Nagai, T Takahashi, H Masuda, Y Shioya… - …, 1988 - rep.bioscientifica.com
In Exp. 1 pig oocytes matured in vitro were used to evaluate the fertilizability in vitro of frozen
epididymal (4 boars) and ejaculated (3 boars) spermatozoa that were preincubated in …
epididymal (4 boars) and ejaculated (3 boars) spermatozoa that were preincubated in …