[HTML][HTML] Comparative analysis between slow freezing and ultra-rapid freezing for human sperm cryopreservation

NS Riva, C Ruhlmann, RS Iaizzo… - JBRA assisted …, 2018 - ncbi.nlm.nih.gov
Objective Cryopreservation of human spermatozoa is fundamental in assisted reproductive
technology. At present, slow freezing techniques are widely used for sperm …

Improved cryopreservation of spermatozoa using vitrification: comparison of cryoprotectants and a novel device for long-term storage

HC O'Neill, M Nikoloska, HT Ho, A Doshi… - Journal of Assisted …, 2019 - Springer
Study question Does cryoprotection of spermatozoa using a vitrification protocol with
improved cryoprotective agents and a novel device for large storage lead to better outcomes …

Cryopreservation of human spermatozoa by vitrification versus conventional rapid freezing: Effects on motility, viability, morphology and cellular defects

MT Le, TTT Nguyen, TT Nguyen, TTA Nguyen… - European Journal of …, 2019 - Elsevier
Objectives Sperm cryopreservation has great potential for male infertility treatment as used
in assisted reproduction technology (ART). There are a variety of cryopreservation methods …

Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation

S Petyim, C Neungton, I Thanaboonyawat… - Journal of assisted …, 2014 - Springer
Purpose To evaluate whether sperm preparation (swim-up technique) before freezing
improves the percentages of sperm motility, sperm viability, and non-apoptotic spermatozoa …

[HTML][HTML] Repeated vitrification/warming of human sperm gives better results than repeated slow programmable freezing

T Vutyavanich, W Lattiwongsakorn… - Asian Journal of …, 2012 - ncbi.nlm.nih.gov
In this study, we compared the effects of repeated freezing/thawing of human sperm by our
in-house method of rapid freezing with slow programmable freezing. Sperm samples from …

Improving native human sperm freezing protection by using a modified vitrification method

D Zhou, XM Wang, RX Li, YZ Wang… - Asian Journal of …, 2021 - journals.lww.com
Slow freezing is the most commonly used technique for the cryopreservation of spermatozoa
in clinical practice. However, it has been shown to have a negative impact on sperm function …

Vitrification is not superior to rapid freezing of normozoospermic spermatozoa: effects on sperm parameters, DNA fragmentation and hyaluronan binding

A Agha-Rahimi, MA Khalili, A Nabi… - Reproductive …, 2014 - Elsevier
Human sperm vitrification is a new cryopreservation method. This study compared the
effects of rapid freezing and vitrification on various sperm parameters, hyaluronan-binding …

Human spermatozoa cryopreservation: comparison of three different freezing protocols.

C Omes, AL Marchetti, ML Masanti, R Bassani… - Cryo …, 2013 - europepmc.org
Human spermatozoa cryopreservation has significantly improved over the last few decades,
but the actual protocols are neither optimal nor standardized between different laboratories …

[HTML][HTML] Cryopreservation of human spermatozoa: comparison of two cryopreservation methods and three cryoprotectants

KP Nallella, RK Sharma, SSR Allamaneni, N Aziz… - Fertility and sterility, 2004 - Elsevier
OBJECTIVE: To evaluate the ability of two cryopreservation methods and three
cryoprotectants to preserve sperm quality. DESIGN: A prospective clinical study. SETTING …

Update on techniques for cryopreservation of human spermatozoa

C Huang, YL Tang, JL Hu, WJ Zhou… - Asian Journal of …, 2022 - journals.lww.com
In the 1960s, sperm cryopreservation was developed as a method to preserve fertility.
Currently, techniques for the cryopreservation of human spermatozoa have been widely …