The genetic engineering of genome fragments larger than 100 kbp is challenging and
requires both specific methods and cloning hosts. The yeast Saccharomyces cerevisiae is …

Over the past decade, a new strategy was developed to bypass the difficulties to genetically
engineer some microbial species by transferring (or “cloning”) their genome into another …

The CRISPR/Cas9 technology has greatly improved genome editing in Saccharomyces
cerevisiae over recent years. However, several current CRISPR/Cas9 systems suffer from …

CRISPR‐Cas9 has emerged as a powerful method for editing the genome in a wide variety
of species, since it can generate a specific DNA break when targeted by the Cas9‐bound …

Until recently, the favored method for making directed modifications to the budding yeast
genome involved the introduction of a DNA template carrying the desired genetic changes …

The yeast S. cerevisiae serves as a model organism for many decades. Numerous
molecular tools have been developed throughout the years to engineer its genome …

Saccharomyces cerevisiae continues to serve as a powerful model system for both basic
biological research and industrial application. The development of genome-wide collections …

One remarkable achievement in synthetic biology was the reconstruction of mycoplasma
genomes and their cloning in yeast where they can be modified using available genetic …

Thanks to its sophistication, the CRISPR/Cas system has been a widely used yeast genome
editing method. However, CRISPR methods generally rely on preassembled DNAs and …

Genome editing using the CRISPR/Cas system has been implemented for various
organisms and becomes increasingly popular even in the genetically tractable budding …