The production of transgenic cattle presents a number of unique challenges not
encountered in other species. First, the survival of microinjected zygotes is low; only 15% in …

Transgenic technology permits major modifications of phenotype by introducing subtle
changes in genotype. For domestic farm species, genetic modification may be used to …

The introduction of specific genes into the genome of farm animals and its stable
incorporation into the germ line has been a major technological advance in agriculture …

The creation of transgenic livestock is a complex multistep procedure the successful
execution of which demands a high level of skill and application. Useful animals have been …

The introduction of foreign DNA into the genome of livestock and its stable integration into
the germ line has been a major technical advance in agriculture. Production of transgenic …

The overall efficiency of somatic cloning in cattle is still low. Many factors are necessary for
successful birth of live offspring. Among them, the source of donor cells reveals the …

Considerable progress has been made in methods for production of transgenic livestock;
beginning with pronuclear microinjection over 20 years ago. New methods, including the …

We examined transgenic‐cattle production by DNA microinjection into 1‐, 2‐, and 4‐cell
embryos, analyzing the impact on calf size and subsequent viability. Embryos were either …

Transgenic research usually involves a large investment in carrying out a series of low-
efficiency steps to produce a few transgenic founder animals, and then characterizing a …

In vitro bovine embryo production (IVP) has improved tremendously during the past decade.
Repeatable and reliable systems for mass production of embryos for use in cattle breeding …