Cell surface display of proteins can be used for several biotechnological applications such
as the screening of protein libraries, whole cell biocatalysis and live vaccine development …

Expressing proteins of interest as fusions to proteins of the bacterial envelope is a powerful
technique with many biotechnological and medical applications. Autotransporters have …

Highlights•Autotransporters have predominantly been used for surface display on E.
coli.•For industrial applications, host bacteria with specific features are needed.•Here we …

The classical monomeric autotransporters are ubiquitously used by Gram-negative bacteria
to export virulence and colonization factors to their cell surface or into their surroundings …

Background Cell display technologies (eg bacterial display) are attractive in directed
evolution as they provide the option to use flow-cytometric cell sorting for selection from …

Escherichia coli is still a very popular host for the production of recombinant proteins at an
analytical or industrial scale. Secretion of the proteins into the culture medium or display at …

Among the pathways used by gram-negative bacteria for protein secretion, the
autotransporter pathway represents a solution of impressive simplicity. Proteins are …

Background Bacterial surface display technique enables the exogenous proteins or
polypeptides displayed on the bacterial surface, while maintaining their relatively …

Autotransporters are widely distributed among Gram-negative bacteria. They can have a
large variety of functions and many of them have a role in virulence. They are synthesized as …

Autotransporters of gram-negative bacteria are single-peptide secretion systems that consist
of a functional N-terminal α-domain (“passenger”) fused to a C-terminal β-domain …