Establishing saturated mutagenesis in a specific gene through gene editing is an efficient
approach for identifying the relationships between mutations and the corresponding …

Current DNA base editors contain nuclease and DNA deaminase that enables deamination
of cytosine (C) or adenine (A), but no method for guanine (G) or thymine (T) editing is …

Background Many favorable traits of crops and livestock and human genetic diseases arise
from multiple single nucleotide polymorphisms or multiple point mutations with …

Deaminase base editing has emerged as a tool to install or correct point mutations in the
genomes of living cells in a wide range of organisms. However, the genome-wide off-target …

Base editors show promise for treating human genetic diseases, but most current systems
use deaminases, which cause off-target effects and are limited in editing type. In this study …

CRISPR base editing techniques tend to edit multiple bases in the targeted region, which is
a limitation for precisely reverting disease-associated single-nucleotide polymorphisms …

About 47% of pathogenic point mutations could be corrected by ABE-induced A· T-to-G· C
conversions. However, the applications of ABEs are still hindered by undesired editing …

Background Base editors are a class of genome editing tools with the ability to efficiently
induce point mutations in genomic DNA, without inducing double-strand breaks or relying on …

Adenine base editors (ABE) are genome-editing tools that have been harnessed to
introduce precise A• T to G• C conversion. However, the low activity of ABE at certain sites …

A recently developed adenine base editor (ABE) efficiently converts A to G and is potentially
useful for clinical applications. However, its precision and efficiency in vivo remains to be …