Several CRISPR-Cas9 orthologues have been used for genome editing. Here, we present
the smallest Cas9 orthologue characterized to date, derived from Campylobacter jejuni …

The repurposed CRISPR–Cas9 system has recently emerged as a revolutionary genome-
editing tool. Here we report a modification in the expression of the guide RNA (gRNA) …

The type-V CRISPR effector Cas12b (formerly known as C2c1) has been challenging to
develop for genome editing in human cells, at least in part due to the high temperature …

Molecular scissors engineered for site-specific modification of the genome hold great
promise for effective functional analyses of genes, genomes and epigenomes and could …

CRISPR-Cas9 nucleases target specific DNA sequences using a guide RNA but also
require recognition of a protospacer adjacent motif (PAM) by the Cas9 protein. Although …

CRISPR–Cas9 is a versatile RNA-guided genome editing tool. Here we demonstrate that
partial replacement of RNA nucleotides with DNA nucleotides in CRISPR RNA (crRNA) …

Advances in the development of delivery, repair, and specificity strategies for the CRISPR-
Cas9 genome engineering toolbox are helping researchers understand gene function with …

CRISPR is becoming an indispensable tool in biological research. Once known as the
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …

The CRISPR-Cas12a system shows unique features compared with widely used Cas9,
making it an attractive and potentially more precise alternative. However, the adoption of this …

CRISPR-Cas genome engineering has revolutionized biomedical research by enabling
targeted genome modification with unprecedented ease. In the popular model organism …