The modem microbiologist is often a real specialist who has difficulty under standing and
applying many of the techniques beyond those in his or her own immediate field. On the …

Light and electron cryo-microscopy have each proven to be powerful tools to study
biological structures in a near-native state. Light microscopy provides important localization …

Cryoelectron microscopy of biological molecules is among the hottest growth areas in
biophysics and structural biology at present, and Frank is arguably the most distinguished …

These continue to be heady times for bacterial cell biology, both for experimentalists and for
modelers. The ability to follow the dynamics of fluorescently labeled proteins has had a huge …

Observing cells and cell components in three dimensions at high magnification in
transmission electron microscopy requires preparing serial ultrathin sections of the …

Recently, significant technical breakthroughs in both hardware equipment and software
algorithms have enabled cryo-electron microscopy (cryo-EM) to become one of the most …

Cryo-electron microscopy (CryoEM) has superseded X-ray crystallography and NMR to
emerge as a popular and effective tool for structure determination in recent times. It has …

Single-particle cryo-electron microscopy (cryo-EM) has emerged over the last two decades
as a technique capable of studying challenging systems that otherwise defy structural …

Single-particle electron cryomicroscopy is undergoing a technical revolution due to the
recent developments of direct detectors. These new recording devices detect electrons …

Most life scientists would agree that understanding how cellular processes work requires
structural knowledge about the macromolecules involved. For example, deciphering the …