Engineering of alkyl-and haloaromatic-responsive gene expression with mini-transposons containing regulated promoters of biodegradative pathways of …

V De Lorenzo, S Fernández, M Herrero, U Jakubzik… - Gene, 1993 - Elsevier
Four recombinant mini-TnJ transposons are described which contain outward-facing Pm, Pu
or Psal promoters from the catabolic plasmids TOL and NAH of Pseudomonas putida, along …

A T7 RNA polymerase-based system for the construction of Pseudomonas strains with phenotypes dependent on TOL-meta pathway effectors

M Herrero, V de Lorenzo, B Ensley, KN Timmis - Gene, 1993 - Elsevier
A general method to construct recombinant Pseudomonas putida (and related bacteria),
which transcribe specific genes inserted into their chromosome in response to the presence …

Analysis of Pseudomonas gene products using lacIq/Ptrp-lac plasmids and transposons that confer conditional phenotypes

V de Lorenzo, L Eltis, B Kessler, KN Timmis - Gene, 1993 - Elsevier
Novel transposon and plasmid-based broad-host-range expression systems have been
developed to facilitate the genetic analysis of gene products of Pseudomonas and related …

VTR expression cassettes for engineering conditional phenotypes in Pseudomonas: activity of the Pu promoter of the TOL plasmid under limiting concentrations of the …

J Pérez-Martin, V de Lorenzo - Gene, 1996 - Elsevier
A simplified procedure to construct recombinant Pseudomonas putida (Pp) and related
bacteria, which transcribe conditionally specific genes inserted into their chromosome in …

Metabolic engineering of bacteria for environmental applications: construction of Pseudomonas strains for biodegradation of 2-chlorotoluene

MA Haro, V de Lorenzo - Journal of Biotechnology, 2001 - Elsevier
In this article, we illustrate the challenges and bottlenecks in the metabolic engineering of
bacteria destined for environmental bioremediation, by reporting current efforts to construct …

Analysis and manipulation of plasmid-encoded pathways for the catabolism of aromatic compounds by soil bacteria

KN Timmis, PR Lehrbach, S Harayama, RH Don… - Plasmids in bacteria, 1985 - Springer
Soil and water bacteria in general, and members of the genus Pseudomonas in particular,
exhibit a fascinating wealth of exotic properties not found in commensal and parasitic …

Cross talk between catabolic pathways in Pseudomonas putida: XylS-dependent and-independent activation of the TOL meta operon requires the same cis-acting …

B Kessler, S Marqués, T Köhler, JL Ramos… - Journal of …, 1994 - Am Soc Microbiol
The Pm promoter of the meta cleavage operon in the TOL (toluene degradation) plasmid
pWW0 of Pseudomonas putida becomes activated by the plasmid-encoded XylS regulator in …

Transcriptional control of the multiple catabolic pathways encoded on the TOL plasmid pWW53 of Pseudomonas putida MT53

MT Gallegos, PA Williams, JL Ramos - Journal of bacteriology, 1997 - Am Soc Microbiol
The TOL plasmid pWW53 encodes a catabolic pathway for the metabolism of toluene. It
bears an upper-pathway operon for the oxidation of toluene to benzoate and a copy of the …

Transcriptional induction kinetics from the promoters of the catabolic pathways of TOL plasmid pWW0 of Pseudomonas putida for metabolism of aromatics

S Marqués, A Holtel, KN Timmis… - Journal of bacteriology, 1994 - Am Soc Microbiol
We determined, under several growth conditions, the kinetics of mRNA synthesis from the
four Pseudomonas putida pWW0 plasmid promoters involved in degradation of xylenes and …

Expression of the TOL plasmid xylS gene in Pseudomonas putida occurs from a alpha 70-dependent promoter or from alpha 70-and alpha 54-dependent tandem …

MT Gallegos, S Marqués, JL Ramos - Journal of bacteriology, 1996 - Am Soc Microbiol
Growth of Pseudomonas putida (pWWO) on alkylbenzoates requires the expression of the
meta pathway operon, which is mediated by the XylS protein after binding of a benzoate …