The solvent-tolerant strain Pseudomonas putida DOT-T1E has been engineered for
biotransformation of toluene into 4-hydroxybenzoate (4-HBA). P. putida DOT-T1E transforms …

Although a wide range of microorganisms have been discovered that are able to degrade
highly stable, toxic xenobiotics, still many pollutants persist in the environment. Recent …

Summary The XylS/Pm regulator/promoter system originating from the Pseudomonas putida
TOL plasmid pWW 0 is widely used for regulated low‐and high‐level recombinant …

Summary Pseudomonas putida DOT‐T1E has the capacity to grow in the presence of high
concentrations of toluene. This ability is mainly conferred by an efflux pump encoded in a …

Unraveling the complex transcriptional regulation of bacterial catabolism of aromatic
pollutants is a prerequisite for engineering efficient biological systems for many …

Cointegrate plasmids were formed in vivo between the broad-host-range R-plasmid RP4
and two catabolic plasmids derived from Pseudomonas putida HS1. One of these was the …

A broad host range, orthogonal genetic platform has been developed to format sensor
circuits in the chromosome of Gram‐negative microorganisms destined for environmental …

Recombinant TOL plasmid pWWO-EB62 allows Pseudomonas putida to grow on p-
ethylbenzoate. This plasmid can be transferred to other microorganisms, and its catabolic …

A DNA segment involved in the regulation of the isopropylbenzene (cumene) catabolism
operon (ipb) of plasmid pRE4 from Pseudomonas putida RE204 and the Vibrio fischeri …

ABSTRACT A 3,372-bp insertion sequence, IS Ppu12, has been identified on the archetypal
toluene-xylene TOL catabolic plasmid pWW0 from Pseudomonas putida mt-2. The insertion …